Systematic analysis of the Capsicum ERF transcription factor family: identification of regulatory factors involved in the regulation of species-specific metabolites

Song, Jiali1; Chen, Changming1,2; Zhang, Shuanglin1; Wang, Juntao1; Huang, Zhubing1; Chen, Muxi1,3; Cao, Bihao1,2; Zhu, Zhangsheng1,2,4; Lei, Jianjun1,2,5

2020-08-24

10.1186/s12864-020-06983-3

BMC GENOMICS   影响因子和分区

1471-2164

BackgroundERF transcription factors (TFs) belong to the Apetala2/Ethylene responsive Factor (AP2/ERF) TF family and play a vital role in plant growth and development processes. Capsorubin and capsaicinoids have relatively high economic and nutritional value, and they are specifically found in Capsicum. However, there is little understanding of how ERFs participate in the regulatory networks of capsorubin and capsaicinoids biosynthesis.ResultsIn this study, a total of 142 ERFs were identified in the Capsicum annuum genome. Subsequent phylogenetic analysis allowed us to divide ERFs into DREB (dehydration responsive element binding proteins) and ERF subfamilies, and further classify them into 11 groups with several subgroups. Expression analysis of biosynthetic pathway genes and CaERFs facilitated the identification of candidate genes related to the regulation of capsorubin and capsaicinoids biosynthesis; the candidates were focused in cluster C9 and cluster C10, as well as cluster L3 and cluster L4, respectively. The expression patterns of CaERF82, CaERF97, CaERF66, CaERF107 and CaERF101, which were found in cluster C9 and cluster C10, were consistent with those of accumulating of carotenoids (beta -carotene, zeaxanthin and capsorubin) in the pericarp. In cluster L3 and cluster L4, the expression patterns of CaERF102, CaERF53, CaERF111 and CaERF92 were similar to those of the accumulating capsaicinoids. Furthermore, CaERF92, CaERF102 and CaERF111 were found to be potentially involved in temperature-mediated capsaicinoids biosynthesis.ConclusionThis study will provide an extremely useful foundation for the study of candidate ERFs in the regulation of carotenoids and capsaicinoids biosynthesis in peppers.

Pepper GENOME-WIDE IDENTIFICATION ERF FUNCTIONAL-ANALYSIS Carotenoids GENE FAMILY Capsaicinoids EXPRESSION Temperature PEPPER FRUIT ARABIDOPSIS TOLERANCE EVOLUTION BIOSYNTHESIS

SCI

英语

通讯

Biotechnology & Applied Microbiology ; Genetics & Heredity

Biotechnology & Applied Microbiology ; Genetics & Heredity

WOS:000567130700002

BMC

MOLECULAR BIOLOGY & GENETICS

1.South China Agr Univ, Coll Hort, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops South, Guangzhou 510642, Guangdong, Peoples R China;
2.Lingnan Guangdong Lab Modern Agr, Guangzhou 510642, Peoples R China;
3.Guangdong Helinong Seeds CO LTD, Shantou 515800, Guangdong, Peoples R China;
4.Southern Univ Sci & Technol, Dept Biol, Peking Univ Southern Univ Sci & Technol Joint Ins, Shenzhen 518055, Peoples R China;
5.Shaoguan Univ, Henry Fok Coll Biol & Agr, Shaoguan 512005, Peoples R China

Song, Jiali,Chen, Changming,Zhang, Shuanglin,et al. Systematic analysis of the Capsicum ERF transcription factor family: identification of regulatory factors involved in the regulation of species-specific metabolites[J]. BMC GENOMICS,2020,21(1).

Song, Jiali.,Chen, Changming.,Zhang, Shuanglin.,Wang, Juntao.,Huang, Zhubing.,...&Lei, Jianjun.(2020).Systematic analysis of the Capsicum ERF transcription factor family: identification of regulatory factors involved in the regulation of species-specific metabolites.BMC GENOMICS,21(1).

Song, Jiali,et al."Systematic analysis of the Capsicum ERF transcription factor family: identification of regulatory factors involved in the regulation of species-specific metabolites".BMC GENOMICS 21.1(2020).