Identification of Histone deacetylase (HDAC)-Associated Proteins with DNA-Programmed Affinity Labeling

Zhang, Jianfu1,2; Peng, Jianzhao1,2,3; Huang, Yiran1,2; Meng, Ling1,2; Li, Qingrong1,2,3; Xiong, Feng1,2; Li, Xiaoyu1,2

2020-08-11

10.1002/anie.202001205

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   影响因子和分区

1433-7851

1521-3773

Histone deacetylase (HDAC) is a major class of deacetylation enzymes. Many HDACs exist in large protein complexes in cells and their functions strongly depend on the complex composition. The identification of HDAC-associated proteins is highly important in understanding their molecular mechanisms. Although affinity probes have been developed to study HDACs, they were mostly targeting the direct binder HDAC, while other proteins in the complex remain underexplored. We report a DNA-based affinity labeling method capable of presenting different probe configurationswithoutthe need for preparing multiple probes. Using one binding probe, 9 probe configurations were created to profile HDAC complexes. Notably, this method identified indirect HDAC binders that may be inaccessible to traditional affinity probes, and it also revealed new biological implications for HDAC-associated proteins. This study provided a simple and broadly applicable method for characterizing protein-protein interactions.

affinity probes ACTIVITY-BASED PROBES DNA-templated synthesis CHEMICAL PROBES histone deacetylases HDAC INHIBITOR photoaffinity labelling BINDING protein-protein interactions GENE TARGETS ACETYLATION SELECTIVITY COMPLEXES DESIGN

SCI ; EI ; IC

英语

NI论文

其他

Research Grants Council of the Hong Kong SAR[AoE/P-705/16][17321916][17302817][17301118][17111319] ; National Natural Science Foundation of China[21572014][21877093][91953119]

Chemistry

Chemistry, Multidisciplinary

WOS:000557983300001

WILEY-V C H VERLAG GMBH

20203309037932

Binders ; Acetylation ; DNA ; Proteins ; Biosynthesis

Biological Materials and Tissue Engineering:461.2 ; Biotechnology:461.8 ; Biochemistry:801.2 ; Chemical Reactions:802.2 ; Chemical Agents and Basic Industrial Chemicals:803 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Univ Hong Kong, Dept Chem, Lab Synthet Chem & Chem Biol Hlth InnoHK, Pokfulam Rd, Hong Kong, Peoples R China;
2.Univ Hong Kong, State Key Lab Synthet Chem, Pokfulam Rd, Hong Kong, Peoples R China;
3.Southern Univ Sci & Technol China, Dept Chem, 1088 Xueyuan Rd, Shenzhen, Peoples R China

Zhang, Jianfu,Peng, Jianzhao,Huang, Yiran,et al. Identification of Histone deacetylase (HDAC)-Associated Proteins with DNA-Programmed Affinity Labeling[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020,59(40):17525-17532.

Zhang, Jianfu.,Peng, Jianzhao.,Huang, Yiran.,Meng, Ling.,Li, Qingrong.,...&Li, Xiaoyu.(2020).Identification of Histone deacetylase (HDAC)-Associated Proteins with DNA-Programmed Affinity Labeling.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,59(40),17525-17532.

Zhang, Jianfu,et al."Identification of Histone deacetylase (HDAC)-Associated Proteins with DNA-Programmed Affinity Labeling".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 59.40(2020):17525-17532.