Coexpression network analysis reveals an MYB transcriptional activator involved in capsaicinoid biosynthesis in hot peppers

Sun，Binmei1; Zhou，Xin1,2; Chen，Changming1; Chen，Chengjie1; Chen，Kunhao3,4; Chen，Muxi3,4; Liu，Shaoqun1; Chen，Guoju1; Cao，Bihao1; Cao，Fanrong1; Lei，Jianjun1,5; Zhu，Zhangsheng1,6

2020-12-01

10.1038/s41438-020-00381-2

Horticulture Research   影响因子和分区

2052-7276

2052-7276

Plant biosynthesis involves numerous specialized metabolites with diverse chemical natures and biological activities. The biosynthesis of metabolites often exclusively occurs in response to tissue-specific combinatorial developmental cues that are controlled at the transcriptional level. Capsaicinoids are a group of specialized metabolites that confer a pungent flavor to pepper fruits. Capsaicinoid biosynthesis occurs in the fruit placenta and combines its developmental cues. Although the capsaicinoid biosynthetic pathway has been largely characterized, the regulatory mechanisms that control capsaicinoid metabolism have not been fully elucidated. In this study, we combined fruit placenta transcriptome data with weighted gene coexpression network analysis (WGCNA) to generate coexpression networks. A capsaicinoid-related gene module was identified in which the MYB transcription factor CaMYB48 plays a critical role in regulating capsaicinoid in pepper. Capsaicinoid biosynthetic gene (CBG) and CaMYB48 expression primarily occurs in the placenta and is consistent with capsaicinoid biosynthesis. CaMYB48 encodes a nucleus-localized protein that primarily functions as a transcriptional activator through its C-terminal activation motif. CaMYB48 regulates capsaicinoid biosynthesis by directly regulating the expression of CBGs, including AT3a and KasIa. Taken together, the results of this study indicate ways to generate robust networks optimized for the mining of CBG-related regulators, establishing a foundation for future research elucidating capsaicinoid regulation.

SCI

英语

通讯

National Key Research and Development Program[2018YFD1000800] ; National Natural Science Foundation of China[31572124]

Plant Sciences ; Genetics & Heredity ; Agriculture

Plant Sciences ; Genetics & Heredity ; Horticulture

WOS:000576936700009

NANJING AGRICULTURAL UNIV

2-s2.0-85091713936

Scopus

1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China),Ministry of Agriculture and Rural Affairs,College of Horticulture,South China Agricultural University,Guangzhou,510642,China
2.Jiangxi Agricultural Engineering College,Zhangshu,331200,China
3.Guangdong Helinong Seeds,Co.,Ltd.,Shantou,515800,China
4.Guangdong Helinong Agricultural Research Institute,Co.,Ltd.,Shantou,515800,China
5.Henry School of Agricultural Science and Engineering,Shaoguan University,Guangdong,512005,China
6.Peking University—Southern University of Science and Technology Joint Institute of Plant and Food Sciences,Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China

Sun，Binmei,Zhou，Xin,Chen，Changming,et al. Coexpression network analysis reveals an MYB transcriptional activator involved in capsaicinoid biosynthesis in hot peppers[J]. Horticulture Research,2020,7(1).

Sun，Binmei.,Zhou，Xin.,Chen，Changming.,Chen，Chengjie.,Chen，Kunhao.,...&Zhu，Zhangsheng.(2020).Coexpression network analysis reveals an MYB transcriptional activator involved in capsaicinoid biosynthesis in hot peppers.Horticulture Research,7(1).

Sun，Binmei,et al."Coexpression network analysis reveals an MYB transcriptional activator involved in capsaicinoid biosynthesis in hot peppers".Horticulture Research 7.1(2020).