Advances and challenges for fluorescence nanothermometry

Zhou，Jiajia1; del Rosal，Blanca2; Jaque，Daniel3,4; Uchiyama，Seiichi5; Jin，Dayong1,6

2020-10-01

10.1038/s41592-020-0957-y

NATURE METHODS   影响因子和分区

1548-7091

1548-7105

Fluorescent nanothermometers can probe changes in local temperature in living cells and in vivo and reveal fundamental insights into biological properties. This field has attracted global efforts in developing both temperature-responsive materials and detection procedures to achieve sub-degree temperature resolution in biosystems. Recent generations of nanothermometers show superior performance to earlier ones and also offer multifunctionality, enabling state-of-the-art functional imaging with improved spatial, temporal and temperature resolutions for monitoring the metabolism of intracellular organelles and internal organs. Although progress in this field has been rapid, it has not been without controversy, as recent studies have shown possible biased sensing during fluorescence-based detection. Here, we introduce the design principles and advances in fluorescence nanothermometry, highlight application achievements, discuss scenarios that may lead to biased sensing, analyze the challenges ahead in terms of both fundamental issues and practical implementations, and point to new directions for improving this interdisciplinary field.

SCI

英语

ESI高被引

其他

Australian Research Council (ARC)[DE180100669][DE200100985] ; Ministerio de Economia y Competitividad-MINECO[MAT2016-75362-C3-1-R] ; Ministerio de Ciencia e Innovacion of Spain[PID2019-106211RB-I00] ; Comunidad de Madrid[B2017/BMD-3867] ; Japan Society for the Promotion of Science[17H03075]

Biochemistry & Molecular Biology

Biochemical Research Methods

WOS:000573463500001

NATURE RESEARCH

BIOLOGY & BIOCHEMISTRY

2-s2.0-85091816710

Scopus

1.Institute for Biomedical Materials & Devices (IBMD),Faculty of Science,University of Technology Sydney,Sydney,Australia
2.ARC Centre of Excellence for Nanoscale Biophotonics,RMIT University,Melbourne,Australia
3.Nanobiology Group,Instituto Ramón y Cajal de Investigación Sanitaria,IRYCIS,Madrid,Spain
4.Fluorescence Imaging Group,Departamento de Física de Materiales–Facultad de Ciencias,Universidad Autónoma de Madrid,Madrid,Spain
5.Graduate School of Pharmaceutical Sciences,The University of Tokyo,Tokyo,Japan
6.Department of Biomedical Engineering,Southern University of Science and Technology,Guangdong,China

Zhou，Jiajia,del Rosal，Blanca,Jaque，Daniel,et al. Advances and challenges for fluorescence nanothermometry[J]. NATURE METHODS,2020,17(10):967-980.

Zhou，Jiajia,del Rosal，Blanca,Jaque，Daniel,Uchiyama，Seiichi,&Jin，Dayong.(2020).Advances and challenges for fluorescence nanothermometry.NATURE METHODS,17(10),967-980.

Zhou，Jiajia,et al."Advances and challenges for fluorescence nanothermometry".NATURE METHODS 17.10(2020):967-980.