A Potential Tumor Suppressor Gene Named miR-508-5p Inhibited the Proliferation and Invasion of Human Melanoma Cells by Targeting KIT

Dang, Lin1; Wang, Yan2; Shi, Cuiping1; Liao, Mengsi1; Sun, Zhaojun1; Fang, Sining1

2020-09-30

10.1177/1533033820951801

TECHNOLOGY IN CANCER RESEARCH & TREATMENT   影响因子和分区

1533-0346

1533-0338

Melanoma is the main death cause of human skin cancer. Increasing evidences demonstrate that microRNAs act as key roles in mediating tumor occurrence and progression. MiR-508-5p has proved to participate in the development of various types of human malignancies. However, the role of miR-508-5p in melanoma remained unclear. Inin vitrostudy, miR-508-5p level in peripheral blood samples of patients with melanoma and human melanoma A375 cells was downregulated compared to that in normal peripheral blood samples or normal human epidermal melanocytes (MHEM). MiR-508-5p overexpression significantly inhibited the cell proliferation, migration and invasion in A375 cells, and thus inhibiting KIT expression at both gene and protein levels. Furthermore, western blot analysis showed miR-508-5p reduced cell proliferation by targeting KIT to modulate RAS/RAF/MEK/ERK pathway. Taken together, we speculated that miR-508-5p functioned as an important suppressor in human melanoma by targeting KIT, suggesting miR-508-5p might be a promising tumor suppressor gene for further target therapies from bench to clinic.

miR-508-5p human melanoma cell KIT RAS RAF MEK ERK pathway

SCI

英语

第一 ; 通讯

Young and Middle-aged Scientific Research Backbone Cultivation Project of Shenzhen People's Hospital[Sykypy201914]

Oncology

Oncology

WOS:000576403700001

SAGE PUBLICATIONS INC

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Dermatol,Affiliated Hosp 1,Clin Med Coll 2, 1017 Dongmen North Rd, Shenzhen, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Pathol,Affiliated Hosp 1,Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China

Dang, Lin,Wang, Yan,Shi, Cuiping,et al. A Potential Tumor Suppressor Gene Named miR-508-5p Inhibited the Proliferation and Invasion of Human Melanoma Cells by Targeting KIT[J]. TECHNOLOGY IN CANCER RESEARCH & TREATMENT,2020,19.

Dang, Lin,Wang, Yan,Shi, Cuiping,Liao, Mengsi,Sun, Zhaojun,&Fang, Sining.(2020).A Potential Tumor Suppressor Gene Named miR-508-5p Inhibited the Proliferation and Invasion of Human Melanoma Cells by Targeting KIT.TECHNOLOGY IN CANCER RESEARCH & TREATMENT,19.

Dang, Lin,et al."A Potential Tumor Suppressor Gene Named miR-508-5p Inhibited the Proliferation and Invasion of Human Melanoma Cells by Targeting KIT".TECHNOLOGY IN CANCER RESEARCH & TREATMENT 19(2020).