南方科技大学知识苑(SUSTech KC): The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing

题名	The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing
作者	Xu，Gang 1; Qi，Furong 1; Li，Hanjie 2; Yang，Qianting 1; Wang，Haiyan 1; Wang，Xin 1 ; Liu，Xiaoju 3; Zhao，Juanjuan 1; Liao，Xuejiao 1; Liu，Yang1 ; Liu，Lei 1 ; Zhang，Shuye 4; Zhang，Zheng 1
通讯作者	Liu，Lei
发表日期	2020-12-01
DOI	10.1038/s41421-020-00225-2
发表期刊	Cell Discovery 影响因子和分区
ISSN	2056-5968
EISSN	2056-5968
卷号	6 期号:1
摘要	Understanding the mechanism that leads to immune dysfunction in severe coronavirus disease 2019 (COVID-19) is crucial for the development of effective treatment. Here, using single-cell RNA sequencing, we characterized the peripheral blood mononuclear cells (PBMCs) from uninfected controls and COVID-19 patients and cells in paired broncho-alveolar lavage fluid (BALF). We found a close association of decreased dendritic cells (DCs) and increased monocytes resembling myeloid-derived suppressor cells (MDSCs), which correlated with lymphopenia and inflammation in the blood of severe COVID-19 patients. Those MDSC-like monocytes were immune-paralyzed. In contrast, monocyte-macrophages in BALFs of COVID-19 patients produced massive amounts of cytokines and chemokines, but secreted little interferons. The frequencies of peripheral T cells and NK cells were significantly decreased in severe COVID-19 patients, especially for innate-like T and various CD8 T cell subsets, compared to healthy controls. In contrast, the proportions of various activated CD4 T cell subsets among the T cell compartment, including Th1, Th2, and Th17-like cells were increased and more clonally expanded in severe COVID-19 patients. Patients’ peripheral T cells showed no sign of exhaustion or augmented cell death, whereas T cells in BALFs produced higher levels of IFNG, TNF, CCL4, CCL5, etc. Paired TCR tracking indicated abundant recruitment of peripheral T cells to the severe patients’ lung. Together, this study comprehensively depicts how the immune cell landscape is perturbed in severe COVID-19.
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
资助项目	Science and Technology Innovation Committee of Shenzhen Municipality[2020A1111350032] ; China Postdoctoral Science Foundation[2020T130122ZX] ; National Natural Science Foundation of China[81700540]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000582179400001
出版者	SPRINGERNATURE
Scopus记录号	2-s2.0-85093074691
来源库	Scopus
引用统计	被引频次[WOS]：153
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/203724
专题	南方科技大学医学院南方科技大学第二附属医院
作者单位	1.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People’s Hospital,The Second Affiliated Hospital,School of Medicine,Southern University of Science and Technology,Shenzhen,518112,China 2.CAS Key Laboratory of Quantitative Engineering Biology,Shenzhen Institute of Synthetic Biology,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China 3.Hepatology Unit,Department of Infectious Diseases,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China 4.Shanghai Public Health Clinical Center,Fudan University,Shanghai,201508,China
第一作者单位	南方科技大学医学院; 南方科技大学第二附属医院
通讯作者单位	南方科技大学医学院; 南方科技大学第二附属医院
第一作者的第一单位	南方科技大学医学院; 南方科技大学第二附属医院
推荐引用方式 GB/T 7714	Xu，Gang,Qi，Furong,Li，Hanjie,et al. The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing[J]. Cell Discovery,2020,6(1).
APA	Xu，Gang.,Qi，Furong.,Li，Hanjie.,Yang，Qianting.,Wang，Haiyan.,...&Zhang，Zheng.(2020).The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing.Cell Discovery,6(1).
MLA	Xu，Gang,et al."The differential immune responses to COVID-19 in peripheral and lung revealed by single-cell RNA sequencing".Cell Discovery 6.1(2020).