Enzyme-Regulated Peptide-Liquid Metal Hybrid Hydrogels as Cell Amber for Single-Cell Manipulation

Liu，Dongdong1,2; Yin，Jiaxiang1; Liang，Sen3; Shi，Wensheng3; Jiang，Xingyu1,4; Gao，Yuan1,2

2020-10-14

10.1021/acsami.0c13334

ACS Applied Materials & Interfaces   影响因子和分区

1944-8244

1944-8252

Current strategies to construct cell-based bioartificial tissues largely remain on a multicell level. Taking cell diversity into account, single-cell manipulation is urgently needed for delicate bioartificial tissue construction. Current single-cell isolation and profiling techniques involve invasive processes and thus are not applicable for single-cell manipulation. Here, we managed to fabricate peptide-liquid metal hybrid hydrogels as "cell ambers" which were suitable for single-cell isolation as well as further handling. The successful preparation of uniform liquid metal nanoparticles allowed the fabrication of peptide-liquid metal hydrogel with excellent recovery property upon mechanical destruction. The alkaline phosphatase-instructed supramolecular self-assembly process allowed the formation of microhydrogel post-filling in the PDMS template. The co-culture of the hydrogel precursor and mammalian cells realized the embedding of cells into elastic hydrogels which were the so-called cell ambers. The cell ambers turned out to be biocompatible and capable of supporting cell survival. Aided with the micro-operating system and a laser scanning confocal microscope, we could arrange these as-prepared 3D single-cell ambers into various patterns as desired. Our strategy provided the possibility to manipulate a single cell, which served as a prototype of cell architecture toward cell-based bioartificial tissue construction.

enzyme hydrogel liquid metal nanoparticle peptide single-cell manipulation

SCI

英语

其他

National Key R&D Program of China[2017YFA0205901] ; NSFC[21675036][51873046]

Science & Technology - Other Topics ; Materials Science

Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000582345700011

AMER CHEMICAL SOC

2-s2.0-85092943966

Scopus

1.CAS Center for Excellence in Nanoscience,CAS Key Laboratory of Biomedical Effects of Nanomaterials and Nanosafety,National Center for Nanoscience and Technology,100190,China
2.Sino-Danish College,University of Chinese Academy of Sciences,100049,China
3.Technical Institute of Physics and Chemistry of the Chinese Academy of Sciences,100190,China
4.Department of Biomedical Engineering,Southern University of Science & Technology,Shenzhen,518055,China

Liu，Dongdong,Yin，Jiaxiang,Liang，Sen,et al. Enzyme-Regulated Peptide-Liquid Metal Hybrid Hydrogels as Cell Amber for Single-Cell Manipulation[J]. ACS Applied Materials & Interfaces,2020,12(41):45807-45813.

Liu，Dongdong,Yin，Jiaxiang,Liang，Sen,Shi，Wensheng,Jiang，Xingyu,&Gao，Yuan.(2020).Enzyme-Regulated Peptide-Liquid Metal Hybrid Hydrogels as Cell Amber for Single-Cell Manipulation.ACS Applied Materials & Interfaces,12(41),45807-45813.

Liu，Dongdong,et al."Enzyme-Regulated Peptide-Liquid Metal Hybrid Hydrogels as Cell Amber for Single-Cell Manipulation".ACS Applied Materials & Interfaces 12.41(2020):45807-45813.