Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse

Mu，Tianhao1,2,3,4; Xu，Liqin5,6,7; Zhong，Yu5,6,8; Liu，Xinyu4,9; Zhao，Zhikun5,6; Huang，Chaoben3; Lan，Xiaofeng3; Lufei，Chengchen4,9; Zhou，Yi4,9; Su，Yixun1,3; Xu，Luang9; Jiang，Miaomiao5,6; Zhou，Hongpo5,6; Lin，Xinxin5,6; Wu，Liang5,6; Peng，Siqi5,6; Liu，Shiping5,6; Brix，Susanne7; Dean，Michael10; Dunn，Norris R.11; Zaret，Kenneth S.12; Fu，Xin Yuan1,2,3,4,9,13; Hou，Yong5,6

2020-12-01

10.1038/s42003-020-01364-8

Communications Biology   影响因子和分区

2399-3642

2399-3642

The liver and gallbladder are among the most important internal organs derived from the endoderm, yet the development of the liver and gallbladder in the early embryonic stages is not fully understood. Using a transgenic Foxa2 reporter mouse line, we performed single-cell full-length mRNA sequencing on endodermal and hepatic cells isolated from ten embryonic stages, ranging from E7.5 to E15.5. We identified the embryonic liver developmental trajectory from gut endoderm to hepatoblasts and characterized the transcriptome of the hepatic lineage. More importantly, we identified liver primordium as the nascent hepatic progenitors with both gut and liver features and documented dynamic gene expression during the epithelial-hepatic transition (EHT) at the stage of liver specification during E9.5–11.5. We found six groups of genes switched on or off in the EHT process, including diverse transcripitional regulators that had not been previously known to be expressed during EHT. Moreover, we identified and revealed transcriptional profiling of gallbladder primordium at E9.5. The present data provides a high-resolution resource and critical insights for understanding the liver and gallbladder development.

SCI

英语

其他

Singapore National Medical Research Council[R-713-000-181-511] ; Ministry of Education[R-713-000-169-112] ; Cancer Science Institute of Singapore (CSI, Singapore)[713001010271] ; Office of Deputy President (DPRT) of National University of Singapore, West China Hospital (Sichuan University, China)[139170082] ; President Fund of SUSTech[22/Y01226237] ; National Natural Science Foundation of China[81672593] ; Natural Science Foundation of Guangdong Province, China[2018A030313379] ; Science, Technology and Innovation Commission of Shenzhen Municipality[GJHZ20180419190827179][KQJSCX20170322143848413]

Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics

Biology ; Multidisciplinary Sciences

WOS:000591682900001

NATURE RESEARCH

2-s2.0-85094959898

Scopus

1.Department of Biochemistry,YLL School of Medicine,National University of Singapore,Singapore,119615,Singapore
2.Laboratory of Human Diseases and Immunotherapies,West China Hospital,Sichuan University,Chengdu,610041,China
3.Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
4.GenEros Biopharma,Hangzhou,310018,China
5.BGI-Shenzhen,Shenzhen,518033,China
6.China National GeneBank,BGI-Shenzhen,Shenzhen,518120,China
7.Department of Biotechnology and Biomedicine,Technical University of Denmark,Soltofts Plads,Kongens Lyngby,2800,Denmark
8.School of Biology and Biological Engineering,South China University of Technology,Guangzhou,510006,China
9.Cancer Science Institute of Singapore,YLL School of Medicine,National University of Singapore,Singapore,117599,Singapore
10.Laboratory of Translational Genomics,Division of Cancer Epidemiology & Genetics,National Cancer Institute,Gaithersburg,United States
11.Endodermal Development and Differentiation Laboratory,Institute of Medical Biology,Agency for Science,Technology and Research (A*STAR),Singapore,138672,Singapore
12.Institute for Regenerative Medicine,University of Pennsylvania,Perelman School of Medicine,Smilow Center for Translation Research,Philadelphia,19104,United States
13.State Key Laboratory of Biotherapy,West China Hospital,Sichuan University,Chengdu,610041,China

Mu，Tianhao,Xu，Liqin,Zhong，Yu,et al. Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse[J]. Communications Biology,2020,3(1).

Mu，Tianhao.,Xu，Liqin.,Zhong，Yu.,Liu，Xinyu.,Zhao，Zhikun.,...&Hou，Yong.(2020).Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse.Communications Biology,3(1).

Mu，Tianhao,et al."Embryonic liver developmental trajectory revealed by single-cell RNA sequencing in the Foxa2eGFP mouse".Communications Biology 3.1(2020).