Metabolic defects in splenic B cell compartments from patients with liver cirrhosis

Huang，Man1,2,3; Liu，Xiaoju4,5; Ye，Haocheng1; Zhao，Xin2; Zhao，Juanjuan1; Liu，Yang1; He，Xiaomeng6; Qu，Mengmeng6; Pan，Jing6; Hou，Baidong3; Cheng，Yongqian6; Liu，Zhenwen7; Li，Zhiwei8; Liu，Lei1,2,9; Sun，Jian4,5; Zhang，Shuye10; Zhang，Zheng1,2,9,11

2020-10-01

10.1038/s41419-020-03060-1

Cell Death & Disease   影响因子和分区

2041-4889

2041-4889

Liver cirrhosis is associated with defective vaccine responses and increased infections. Dysregulated B cell compartments in cirrhotic patients have been noticed but not well characterized, especially in the spleen. Here, we comprehensively investigated B cell perturbations from the spleens and peripheral blood of cirrhotic patients. We found that liver cirrhosis significantly depleted both switched and nonswitched splenic memory B cells, which was further confirmed histologically. Bulk RNA-seq revealed significant metabolic defects as the potential mechanism for the impaired splenic B cell functions. Functionally, the splenic memory B cells from cirrhotic patients showed strong metabolic defects and reduced proliferation compared with those from healthy controls. Thus, liver cirrhosis extensively disturbs the splenic and peripheral B cell compartments, which may contribute to defective humoral immunity during liver cirrhosis.

SCI

英语

通讯

National Science and Technology Major Project of the Infectious Diseases[2018ZX10301404][2017ZX10202202] ; National Natural Science Foundation of China[81974259][81871668][81672037] ; Science and Technology Innovation Committee of Shenzhen Municipality[JCYJ20170412151722110][JCYJ20170412151650600] ; Guangzhou Science and Technology Plan Project[201804020001] ; Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program[2017BT01S131]

Cell Biology

Cell Biology

WOS:000586519200005

SPRINGERNATURE

2-s2.0-85093967945

Scopus

1.Institute for Hepatology,National Clinical Research Center for Infectious Disease,Shenzhen Third People’s Hospital,Shenzhen,518112,China
2.The Second Affiliated Hospital,School of Medicine,South University of Science and Technology,Shenzhen,518100,China
3.Institute of Biophysics,Chinese Academy of Sciences,Beijing,100101,China
4.Department of Infectious Diseases,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China
5.State Key Laboratory of Organ Failure Research,Guangdong Provincial Key Laboratory of Viral Hepatitis Research,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China
6.Research Center for Clinical & Translational Medicine,Fifth Medical Center for General Hospital of PLA,Beijing,100039,China
7.Department for Liver Transplantation,Fifth Medical Center for General Hospital of PLA,Beijing,100039,China
8.Department for Liver Transplantation,Shenzhen Third People’s Hospital,Shenzhen,518112,China
9.Guangdong Key Lab of Emerging Infectious Diseases,Shenzhen Third People’s Hospital,Shenzhen,518112,China
10.Shanghai Public Health Clinical Center,Fudan University,Shanghai,201508,China
11.Institute of Hepatology,Shenzhen 3rd People’s Hospital,Shenzhen,5181112,China

Huang，Man,Liu，Xiaoju,Ye，Haocheng,et al. Metabolic defects in splenic B cell compartments from patients with liver cirrhosis[J]. Cell Death & Disease,2020,11(10).

Huang，Man.,Liu，Xiaoju.,Ye，Haocheng.,Zhao，Xin.,Zhao，Juanjuan.,...&Zhang，Zheng.(2020).Metabolic defects in splenic B cell compartments from patients with liver cirrhosis.Cell Death & Disease,11(10).

Huang，Man,et al."Metabolic defects in splenic B cell compartments from patients with liver cirrhosis".Cell Death & Disease 11.10(2020).