Fluorescence Sensor Array for Discrimination of Urine Proteins and Differentiation Diagnosis of Urinary System Diseases

Shen，Yishun1,2; Huang，Yuanfang1,2; Zhang，Pangmiaomiao1; Guo，Baochun3; Jiang，Hongtao4; Tan，Chunyan1,2; Jiang，Yuyang1,5

2020-09-21

10.1021/acsabm.0c00845

ACS Applied Bio Materials   影响因子和分区

2576-6422

Successful discrimination of complex biological samples by sensor array remains a challenging problem. Herein, a three-unit fluorescence sensor array was fabricated using fluorescent molecules, which interact with proteins and generate rich response signals, to realize the discrimination of urinary proteins. As a proof of concept, six urinary proteins were selected as analytes. Results showed 100% accuracy in differentiation and quantitative discrimination of every single protein and their complex mixtures. Moreover, protein profiles of urine samples from healthy people and patients with different urinary diseases, including tubular injury (TI), diabetic nephropathy (DN), and nephropathy (Nep), were well distinguished.

fluorescence sensor array hierarchical clustering analysis (HCA) linear discriminant analysis (LDA) protein urinary system diseases

ESCI ; EI

英语

其他

2-s2.0-85093686393

Scopus

1.State Key Laboratory of Chemical Oncogenomics,Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China
2.Open FIESTA,Shenzhen International Graduate School,Tsinghua University,Shenzhen,518055,China
3.Department of Nephrology,Shenzhen People's Hospital,Second Clinical Medical College,Jinan University,First Affiliated Hospital,Southern University of Science and Technology,Shenzhen, Guangdong,518020,China
4.Department of Urology,Shenzhen Medical Engineering and Technology Center of Minimally Invasive Urology,Shenzhen People's Hospital,Second Clinical Medical College,Jinan University,First Affiliated Hospital,Southern University of Science and Technology,Shenzhen, Guangdong,518020,China
5.School of Pharmaceutical Sciences,Tsinghua University,Beijing,100084,China

Shen，Yishun,Huang，Yuanfang,Zhang，Pangmiaomiao,et al. Fluorescence Sensor Array for Discrimination of Urine Proteins and Differentiation Diagnosis of Urinary System Diseases[J]. ACS Applied Bio Materials,2020,3(9):5639-5643.

Shen，Yishun.,Huang，Yuanfang.,Zhang，Pangmiaomiao.,Guo，Baochun.,Jiang，Hongtao.,...&Jiang，Yuyang.(2020).Fluorescence Sensor Array for Discrimination of Urine Proteins and Differentiation Diagnosis of Urinary System Diseases.ACS Applied Bio Materials,3(9),5639-5643.

Shen，Yishun,et al."Fluorescence Sensor Array for Discrimination of Urine Proteins and Differentiation Diagnosis of Urinary System Diseases".ACS Applied Bio Materials 3.9(2020):5639-5643.