Toxicity and accumulation of 6-OH-BDE-47 and newly synthesized 6,6 '-diOH-BDE-47 in early life-stages of Zebrafish (Danio rerio)

Zhang，Mengtao1,2; Zhao，Feng3; Zhang，Jiawei1; Shi，Jianghong1; Tao，Huanyu1; Ge，Hui1; Guo，Wei1; Liu，Dong3; Cai，Zongwei2

2021-04-01

10.1016/j.scitotenv.2020.143036

SCIENCE OF THE TOTAL ENVIRONMENT   影响因子和分区

0048-9697

1879-1026

Dihydroxylated polybrominated diphenyl ethers (diOH-PBDEs) appear to be natural products or metabolites of PBDEs in some marine organisms, yet its toxicity is still largely unknown. With a newly lab-synthesized diOH-PBDE, 6,6'-dihydroxy-2,2',4'4'-tetrabromodiphenyl ether (6,6'-diOH-BDE-47) in hand, the present study has provided the first data set to compare 6-hydroxy-2,2',4'4'- tetrabromodiphenyl ether (6-OH-BDE-47) and 6,6'-diOH-BDE-47 for their acute toxicity and accumulation, and thyroid hormone levels in treated zebrafish larvae. By real time-PCR technique, transcripts of hypothalamic-pituitary-thyroid axis associated genes were also investigated in developing larvae at 96 h post fertilization (96 hpf). Apparently, 6,6'-diOH-BDE-47 was less toxic than that of 6-OH-BDE-47: 1) the 96-h LC50 (96-h median lethal concentration) of 6-OH-BDE-47 and 6,6'-diOH-BDE-47 were 235 nM and 516 nM, respectively; 2) although severe developmental delays and morphological deformities were observed in zebrafish larvae in high exposure doses, at the exposure concentration of 1-50 nM, the accumulated 6-OH-BDE-47 and 6,6'-diOH-BDE-47 is ranged between 226-2279 nmol/g and 123-539 nmol/g in treated larvae; and 3) for 6-OH-BDE-47, its bioconcentration factor (BCF) were 1.83- to 4.30-fold more than that of 6,6'-diOH-BDE-47, suggesting that the lower internal exposure concentration of 6,6'-diOH-BDE-47 may lead to lower toxicity. The increased thyroid hormone levels were recorded for 1 nM of 6-OH-BDE-47 and 20 nM of 6,6'-diOH-BDE-47, and the exposures both significantly increased thyroid gland-specific transcription of thyroglobulin gene, indicating an adverse effect associated with the HPT axis. Therefore, 6,6'-diOH-BDE-47, with lower toxicity compared to that of 6-OH-BDE-47, still possesses hazards and environmental risk. (C) 2020 Elsevier B.V. All rights reserved.

DiOH-PBDEs Zebrafish Developmental delay Thyroid hormone Gene transcription

SCI ; EI

英语

第一 ; 通讯

Ministry of Science and Technology of the People's Republic of China[2018YFC1801603] ; National Natural Science Foundation of China[21976079,41977325,31771618] ; Shenzhen Science and Technology Innovation Commission (STIC) Project["JCYJ 20170817110953833","JCYJ20190809171201660","JCYJ20180302174233348"]

Environmental Sciences & Ecology

Environmental Sciences

WOS:000608188700094

ELSEVIER

20204509448931

Transcription ; Metabolites ; Ethers ; Polymerase chain reaction ; Hormones ; Organic pollutants ; Marine biology

Biological Materials and Tissue Engineering:461.2 ; Health Care:461.7 ; Biology:461.9 ; Biochemistry:801.2 ; Organic Compounds:804.1

ENVIRONMENT/ECOLOGY

Web of Science

1.State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control,School of Environmental Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
2.China State Key Laboratory of Environmental and Biological Analysis,Department of Chemistry,Hong Kong Baptist University,Hong Kong
3.Department of Biology,Southern University of Science and Technology,Shenzhen,China

Zhang，Mengtao,Zhao，Feng,Zhang，Jiawei,et al. Toxicity and accumulation of 6-OH-BDE-47 and newly synthesized 6,6 '-diOH-BDE-47 in early life-stages of Zebrafish (Danio rerio)[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2021,763.

Zhang，Mengtao.,Zhao，Feng.,Zhang，Jiawei.,Shi，Jianghong.,Tao，Huanyu.,...&Cai，Zongwei.(2021).Toxicity and accumulation of 6-OH-BDE-47 and newly synthesized 6,6 '-diOH-BDE-47 in early life-stages of Zebrafish (Danio rerio).SCIENCE OF THE TOTAL ENVIRONMENT,763.

Zhang，Mengtao,et al."Toxicity and accumulation of 6-OH-BDE-47 and newly synthesized 6,6 '-diOH-BDE-47 in early life-stages of Zebrafish (Danio rerio)".SCIENCE OF THE TOTAL ENVIRONMENT 763(2021).