Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3

Liao，Guixiang1,3; Zhao，Zhihong2; Yang，Hongli1,3; Li，Xiaming1,3

2020

10.1177/0300060520963993

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH   影响因子和分区

0300-0605

1473-2300

Objective: Sirtuin 3 (SIRT3) plays a vital role in regulating oxidative stress in tissue injury. The aim of this study was to evaluate the radioprotective effects of honokiol (HKL) in a zebrafish model of radiation-induced brain injury and in HT22 cells. Methods: The levels of reactive oxygen species (ROS), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β) were evaluated in the zebrafish brain and HT22 cells. The expression levels of SIRT3 and cyclooxygenase-2 (COX-2) were measured using western blot assays and real-time polymerase chain reaction (RT-PCR). Results: HKL treatment attenuated the levels of ROS, TNF-α, and IL-1β in both the in vivo and in vitro models of irradiation injury. Furthermore, HKL treatment increased the expression of SIRT3 and decreased the expression of COX-2. The radioprotective effects of HKL were achieved via SIRT3 activation. Conclusions: HKL attenuated oxidative stress and pro-inflammatory responses in a SIRT3-dependent manner in radiation-induced brain injury.

cyclooxygenase-2 Honokiol ionizing irradiation radiation-induced brain injury reactive oxygen species sirtuin 3

SCI

英语

第一 ; 通讯

Shenzhen Health and Family Planning System Research[SZBC2017024] ; Natural Science Foundation of Shenzhen[JCYJ20170307095828424] ; Cultivation Research for the Youth of Shenzhen People's Hospital[SYKYPY2019029]

Research & Experimental Medicine ; Pharmacology & Pharmacy

Medicine, Research & Experimental ; Pharmacology & Pharmacy

WOS:000585140200001

SAGE PUBLICATIONS LTD

CLINICAL MEDICINE

2-s2.0-85093822942

Scopus

1.Department of Radiation Oncology,Shenzhen People's Hospital,the First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,China
2.Department of Nephrology,Shenzhen People's Hospital,Second Clinical Medicine College of Jinan University,Shenzhen,China
3.Second Clinical Medicine College of Jinan University,Shenzhen,China

Liao，Guixiang,Zhao，Zhihong,Yang，Hongli,et al. Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3[J]. JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,2020,48(10).

Liao，Guixiang,Zhao，Zhihong,Yang，Hongli,&Li，Xiaming.(2020).Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3.JOURNAL OF INTERNATIONAL MEDICAL RESEARCH,48(10).

Liao，Guixiang,et al."Honokiol ameliorates radiation-induced brain injury via the activation of SIRT3".JOURNAL OF INTERNATIONAL MEDICAL RESEARCH 48.10(2020).