Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System

Sun，Kai1; Ding，Zhaoyang1; Zhang，Jicheng1; Chen，Haobin1; Qin，Yuling1; Xu，Shihan1; Wu，Changfeng2; Yu，Jiangbo1; Chiu，Daniel T.1

2020

10.1002/adhm.202001019

Advanced Healthcare Materials   影响因子和分区

2192-2640

2192-2659

Impaired glucose metabolism in diabetes causes severe acute and long-term complications, making real-time detection of blood glucose indispensable for diabetic patients. Existing continuous glucose monitoring systems are unsuitable for long-term clinical glycemic management due to poor long-term stability. Polymer dot (Pdot) glucose transducers are implantable optical nanosensors that exhibit excellent brightness, sensitivity, selectivity, and biocompatibility. Here, it is shown that hydrogen peroxide—a product of glucose oxidation in Pdot glucose sensors—degrades sensor performance via photobleaching, reduces glucose oxidase activity, and generates cytotoxicity. By adding catalase to a glucose oxidase-based Pdot sensor to create an enzymatic cascade, the hydrogen peroxide product of glucose oxidation is rapidly decomposed by catalase, preventing its accumulation and improving the sensor's photostability, enzymatic activity, and biocompatibility. Thus, a next-generation Pdot glucose transducer with a multienzyme reaction system (Pdot–GOx/CAT) that provides excellent sensing characteristics as well as greater detection system stability is presented. Pdot glucose transducers that incorporate this enzymatic cascade to eliminate hydrogen peroxide will possess greater long-term stability for improved continuous glucose monitoring in diabetic patients.

continuous glucose monitoring diabetes enzymatic cascade long-term stability semiconductor polymer dots

SCI ; EI

英语

其他

National Institutes of Health[R01MH113333] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930] ; National Key Research and Development Program of China[2018YFB0407200]

Engineering ; Science & Technology - Other Topics ; Materials Science

Engineering, Biomedical ; Nanoscience & Nanotechnology ; Materials Science, Biomaterials

WOS:000581028700001

WILEY

20204309394359

Oxidation ; Glucose oxidase ; System stability ; Continuous time systems ; Glucose sensors ; Biocompatibility ; Hydrogen peroxide ; Photobleaching ; Transducers

Biology:461.9 ; Immunology:461.9.1 ; Biomedical Equipment, General:462.1 ; Light/Optics:741.1 ; Chemistry:801 ; Chemical Reactions:802.2 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Systems Science:961

2-s2.0-85093533280

Scopus

1.Department of Chemistry,University of Washington,Seattle,98195,United States
2.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Sun，Kai,Ding，Zhaoyang,Zhang，Jicheng,et al. Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System[J]. Advanced Healthcare Materials,2020,10(4).

Sun，Kai.,Ding，Zhaoyang.,Zhang，Jicheng.,Chen，Haobin.,Qin，Yuling.,...&Chiu，Daniel T..(2020).Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System.Advanced Healthcare Materials,10(4).

Sun，Kai,et al."Enhancing the Long-Term Stability of a Polymer Dot Glucose Transducer by Using an Enzymatic Cascade Reaction System".Advanced Healthcare Materials 10.4(2020).