Microfluidic model of the blood-retinal-barrier for permeability tests

Liu，Sihan1,2; Chan，Yau Kei2; Shum，Ho Chueng2; Park，Jaewon1

2019

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

412-413

Compared to commonly used in vitro blood-retinal-barrier (BRB) models that utilize transwells to establish barriers, a microfluidic-based BRB model provides more in vivo-like microenvironments to prompt the formation of tight junctions (TJs) for permeability screening of drug candidates. Here, we developed a microfluidic-based BRB model system for drug permeability study. An in vitro BRB model is built by co-culturing retina pigment epithelial (RPE) cells and choroid endothelial cells on a porous polycarbonate membrane, where the measured trans-epithelial-endothelial resistance (TEER) of the barrier increased up to 45 kΩ•mm. This device is believed to bring an applicable strategy for in vitro ophthalmic drug permeability tests.

Blood-retinal-barrier Microfluidics Organs-on-a-Chip Trans-epithelial-endothelial resistance

第一 ; 通讯

英语

EI

20204509456431

Blood ; Microfluidics ; Aldehydes ; Ophthalmology

Biological Materials and Tissue Engineering:461.2 ; Medicine and Pharmacology:461.6 ; Biology:461.9 ; Microfluidics:632.5.1 ; Organic Compounds:804.1

2-s2.0-85094961907

Scopus

1.Southern University of Science and Technology,Shenzhen,China
2.University of Hong Kong,Hong Kong

Liu，Sihan,Chan，Yau Kei,Shum，Ho Chueng,et al. Microfluidic model of the blood-retinal-barrier for permeability tests[C],2019:412-413.