Rapid identification of KL49 Acinetobacter baumannii associated with clinical mortality

Deng，Qiuyang1; Zhang，Jinyong2; Zhang，Min1; Liu，Zhou3; Zhong，Yuxin1; Liu，Shiyi1; Cui，Ruiqin1; Shi，Yun2; Zeng，Hao2; Yang，Xiyao3; Lin，Chuchu4; Luo，Yutian5; Chen，Huaisheng5; Wu，Weiyuan6; Wu，Jinsong6; Zhang，Tianle7; Lu，Yuemei6; Liu，Xueyan5; Zou，Quanming2; Huang，Wei1

2020

10.2147/IDR.S278891

Infection and Drug Resistance   影响因子和分区

1178-6973

1178-6973

Objective: We aimed to establish a tool for rapid identification of KL49 Acinetobacter baumannii. Methods: Based on the capsular polysaccharide (CPS) synthesis genes database, we investigated the distribution of K locus type 49 (KL49) genes in other KL types and established a rapid identification method for KL49. We collected 61 clinical carbapenem-resistant A. baumannii (CRAB) strains, identified KL49 by gtr100 detection, and used whole genome sequencing (WGS) for verification. A mouse pneumonia model was used to confirm the hypervirulence phenotype. We tested the presence of gtr100 gene in 165 CRAB strains from three provinces in China and evaluated the correlation of gtr100 carrying CRAB infection with mortality. Results: The gtr100 gene is the CPS synthesis gene found only in KL49. We screened out nine WGS-validated KL49 strains from 61 CRAB clinical strains using polymerase chain reaction (PCR) to detect the gtr100 gene. The survival rates of KL49 strains were significantly lower than nonKL49 strains in a mouse pneumonia model. The survival rates of LAC4 gtr100 knockout strain decreased significantly. Analysis of phylogenetics showed the worldwide spread of KL49 A. baumannii. Infection of gtr100 carrying CRAB is an independent risk for mortality (OR, 10.76; 95%CI: 3.08–37.55; p<0.001). Conclusion: The hypervirulence phenotype of KL49 CRAB and the association with mortality highlight the urgent need for implementing control measures. The rapid identification assay has the potential to facilitate early medical intervention and worldwide surveillance.

Acinetobacter baumannii Hypervirulent K locus Kaptive Mortality

SCI

英语

第一

International Collaborative Research Fund of Shenzhen Science and Technology Innovation Commission[GJHZ20180413181716797] ; Free Inquiry Fund of Shenzhen Science and Technology Innovation Commission[JCYJ20180305163929948]

Infectious Diseases ; Pharmacology & Pharmacy

Infectious Diseases ; Pharmacology & Pharmacy

WOS:000590719300005

DOVE MEDICAL PRESS LTD

2-s2.0-85096050797

Scopus

1.Bacteriology & Antibacterial Resistance Surveillance Laboratory,Shenzhen Institute of Respiratory Diseases,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China
2.National Engineering Research Center of Immunological Products,Department of Microbiology and Biochemical Pharmacy,College of Pharmacy,Army Medical University,Chongqing,China
3.Second Affiliated Hospital of Anhui Medical University,Hefei,China
4.Department of Nosocomial Infection Control,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China
5.Intensive Care Unit,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China
6.Department of Clinical Microbiology,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,518020,China
7.Guangdong Medical University,Dongguan,China

Deng，Qiuyang,Zhang，Jinyong,Zhang，Min,et al. Rapid identification of KL49 Acinetobacter baumannii associated with clinical mortality[J]. Infection and Drug Resistance,2020,13:4125-4132.

Deng，Qiuyang.,Zhang，Jinyong.,Zhang，Min.,Liu，Zhou.,Zhong，Yuxin.,...&Huang，Wei.(2020).Rapid identification of KL49 Acinetobacter baumannii associated with clinical mortality.Infection and Drug Resistance,13,4125-4132.

Deng，Qiuyang,et al."Rapid identification of KL49 Acinetobacter baumannii associated with clinical mortality".Infection and Drug Resistance 13(2020):4125-4132.