Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway

Liu，Xin1; Bian，Hong2; Dou，Qing Li1; Huang，Xian Wen1; Tao，Wu Yuan1; Liu，Wen Hua1; Li，Na1; Zhang，Wen Wu1

2020

10.1155/2020/1928410

Biomed Research International   影响因子和分区

2314-6133

2314-6141

Ginkgetin, the extract of Ginkgo biloba leaves, has been reported to exert preventive and therapeutic effects on cardiovascular disease. However, little is known about its role in myocardial ischemia-reperfusion injury (MIRI). The present study aimed to unveil the function of ginkgetin in cardiomyocytes subjected to hypoxia/reoxygenation (H/R) injury. Cell Counting Kit-8 (CCK-8) was employed to evaluate the impact of ginkgetin on cell viability in the absence or presence of H/R. Proinflammatory cytokines and malondialdehyde (MDA), reactive oxygen species (SOD), and lactate dehydrogenase (LDH) were determined via corresponding kits. In addition, flow cytometry was performed to detect apoptotic level. Western blot analysis was utilized to estimate caspase-3 and cytochrome C. Ginkgetin had no significant effect on cell viability; however, it could enhance viability of H9C2 cells exposed to H/R. Inflammation and oxidative stress induced by H/R injury were relieved via pretreatment with ginkgetin. Preconditioning of ginkgetin also decreased apoptotic rate and the protein levels of caspase-3, cytochrome C under H/R condition. Furthermore, 2-HBA, an inducer of caspase-3, was used for the activation of caspase-3 signaling pathway. It was found that induction of caspase-3 eliminated the protective effect of ginkgetin on H9C2 cells exposed to H/R. These results indicated that ginkgetin attenuated inflammation, oxidative stress, and apoptosis. These protective roles of ginkgetin may attribute to caspase-3 dependent pathway.

SCI

英语

其他

Medical and health basic research project of Shenzhen City[2019JD063]

Biotechnology & Applied Microbiology ; Research & Experimental Medicine

Biotechnology & Applied Microbiology ; Medicine, Research & Experimental

WOS:000594097700013

HINDAWI LTD

2-s2.0-85096348307

Scopus

1.Department of Emergency Medicine,Baoan Hospital Affiliated with Southern Medical University,People's Hospital of Baoan District of Shenzhen,China
2.Department of Cardiothoracic Surgery,Southern University of Science and Technology Hospital,China

Liu，Xin,Bian，Hong,Dou，Qing Li,et al. Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway[J]. Biomed Research International,2020,2020.

Liu，Xin.,Bian，Hong.,Dou，Qing Li.,Huang，Xian Wen.,Tao，Wu Yuan.,...&Zhang，Wen Wu.(2020).Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway.Biomed Research International,2020.

Liu，Xin,et al."Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway".Biomed Research International 2020(2020).