In vivo study of polyurethane and tannin-modified hydroxyapatite composites for calvarial regeneration

Tian，Xinggui1,2; Yuan，Xiaowei1,3; Feng，Daxiong2; Wu，Min1; Yuan，Yuping4; Ma，Chuying5; Xie，Denghui1; Guo，Jinshan1; Liu，Chao5; Lu，Zhihui1

2020

10.1177/2041731420968030

Journal of Tissue Engineering   影响因子和分区

2041-7314

2041-7314

Biomaterial mediated bone regeneration is an attractive strategy for bone defect treatment. Organic/inorganic composites have been well established as effective bone graft. Here, the bone regenerative effect of the composites made from tannic acid (TA) modified hydroxyapatite (HA) (THA) or TA & silver nanoparticles (Ag NPs) modified HA (Ag-THA) and polyurethane (PU) was evaluated on critical-sized calvarial defects in rats. The in vivo study indicates that PU/THA and PU/Ag-THA scaffolds exhibited acceptable biocompatibility and induced significantly enhanced bone mineral densities comparing with the blank control (CON) group as well as PU/HA group. The inclusion of TA on HA brought the composites with enhanced osteogenesis and angiogenesis, evidenced by osteocalcin (OCN) and vascular endothelial growth factor (VEGF) immunohistochemical staining. Tartrate resistant acid phosphatase (TRAP) staining showed high osteoclast activity along with osteogenesis, especially in PU/THA and PU/Ag-THA groups. However, further introduction of Ag NPs on HA depressed the angiogenesis of the composites, leading to even lower VEGF expression than that of CON group. This study once more proved that THA can serve as a better bone composite component that pure HA and can promote osteogenesis and angiogenesis. While, the introduction of antimicrobial Ag NPs on HA need to be controlled in some extent not to affect the angiogenesis of the composites.

angiogenesis bone regeneration composite polyurethane Tannin

SCI ; EI

英语

其他

20215011305864

Biocompatibility ; Bone ; Defects ; Flavonoids ; Phosphatases ; Polyurethanes ; Scaffolds ; Scaffolds (biology) ; Silver compounds ; Silver nanoparticles ; Tannins

Construction Equipment:405.1 ; Biomedical Engineering:461.1 ; Biological Materials and Tissue Engineering:461.2 ; Immunology:461.9.1 ; Nanotechnology:761 ; Chemical Products Generally:804 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Organic Polymers:815.1.1 ; Materials Science:951

2-s2.0-85096309813

Scopus

1.Department of Histology and Embryology,School of Basic Medical Sciences,Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases,The Third Affiliated Hospital of Southern Medical University,Southern Medical University,Guangzhou,China
2.Department of Orthopaedics,The Affiliated Hospital of Southwest Medical University,Luzhou,China
3.Department of Orthopedics,Shengjing Hospital of China Medical University,Shenyang,China
4.Department of Material Science and Engineering,Southern University of Science and Technology,Shenzhen,China
5.Aleo BME,Inc.,State College,United States

Tian，Xinggui,Yuan，Xiaowei,Feng，Daxiong,et al. In vivo study of polyurethane and tannin-modified hydroxyapatite composites for calvarial regeneration[J]. Journal of Tissue Engineering,2020,11.

Tian，Xinggui.,Yuan，Xiaowei.,Feng，Daxiong.,Wu，Min.,Yuan，Yuping.,...&Lu，Zhihui.(2020).In vivo study of polyurethane and tannin-modified hydroxyapatite composites for calvarial regeneration.Journal of Tissue Engineering,11.

Tian，Xinggui,et al."In vivo study of polyurethane and tannin-modified hydroxyapatite composites for calvarial regeneration".Journal of Tissue Engineering 11(2020).