南方科技大学知识苑(SUSTech KC): The intervention mechanism of emodin on TLR3 pathway in the process of central nervous system injury caused by herpes virus infection

题名	The intervention mechanism of emodin on TLR3 pathway in the process of central nervous system injury caused by herpes virus infection
作者	Huang，Yongqian 1; Li，Xing 1; Pan，Chunlian 1; Cheng，Wei 1; Wang，Xijia 1; Yang，Zhigang 1; Zheng，Lifang 2
通讯作者	Zheng，Lifang
发表日期	2021-04-03
DOI	10.1080/01616412.2020.1853989
发表期刊	NEUROLOGICAL RESEARCH 影响因子和分区
ISSN	0161-6412
EISSN	1743-1328
卷号	43 期号:4
摘要	["Background and purpose: To investigate the effect of Emodin on the inflammatory response of brain tissue and the expression of the TLR3 pathway in mice with herpes virus encephalitis.","Method: Twenty male BALB/c mice were randomly divided into the NS group, HSV-1 group, HSV-1 + Emodin group and HSV-1 + ACV group. The histopathological features and the effect of TLR3 expression were observed by staining and immunohistochemistry (IHC) respectively. The gene expression of TLR3, trif, TRADD, TRAF6, traf3, p38, Nemo and IRF3 was detected by polymerase chain reaction (PCR). The protein production of TLR3 and its downstream molecules was detected by Western blot. The expression of IL-6, TNF-alpha and IFN-beta in the brain tissues was detected by ELISA.","Result: Compared to the HSV-1 group, the pathological changes (inflammatory cell infiltration, necrotic temporal lobe and massive hemorrhage) were not as obvious as those in the HSV-1+emodin and HSV-1+ACV groups. The TLR3 staining increased significantly in the HSV-1 groups and decreased in the HSV-1 + emodin group. Compared with the NS group, the mRNA expression of TLR3, TRIF, TRADD, TRAF6, traf3, p38, NEMO and IRF3 decreased by 20%-60% in the HSV-1 + emodin group and 30% in the HSV-1 + ACV group, respectively. The expression of IL-6, TNF-alpha and IFN-beta decreased by 30%-50% in the HSV-1 + emodin group and showed no significant change in the HSV-1 + ACV group, respectively.","Conclusion: Emodin could inhibit the inflammatory response in the brain of mice with herpes virus encephalitis. The inhibition of TLR3 expression may play an important role in this process."]
关键词	Emodin TLR3 herpes virus encephalitis
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	通讯
资助项目	2019 Wuhan Medical Scientific Research Program grant[WX19D01]
WOS研究方向	Neurosciences & Neurology
WOS类目	Clinical Neurology ; Neurosciences
WOS记录号	WOS:000596747900001
出版者	TAYLOR & FRANCIS LTD
ESI学科分类	NEUROSCIENCE & BEHAVIOR
来源库	Web of Science
引用统计	被引频次[WOS]：9
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/209714
专题	南方科技大学
作者单位	1.Department of Neurology,Puren Hospital,Wuhan University of Science and Technology,Wuhan,China 2.Department of Neurology,Yantian Hospital of Southern University of Science and Technology,Shenzhen,China
通讯作者单位	南方科技大学
推荐引用方式 GB/T 7714	Huang，Yongqian,Li，Xing,Pan，Chunlian,et al. The intervention mechanism of emodin on TLR3 pathway in the process of central nervous system injury caused by herpes virus infection[J]. NEUROLOGICAL RESEARCH,2021,43(4).
APA	Huang，Yongqian.,Li，Xing.,Pan，Chunlian.,Cheng，Wei.,Wang，Xijia.,...&Zheng，Lifang.(2021).The intervention mechanism of emodin on TLR3 pathway in the process of central nervous system injury caused by herpes virus infection.NEUROLOGICAL RESEARCH,43(4).
MLA	Huang，Yongqian,et al."The intervention mechanism of emodin on TLR3 pathway in the process of central nervous system injury caused by herpes virus infection".NEUROLOGICAL RESEARCH 43.4(2021).