Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa

Vidaillac, Celine1,2,17,18; Yong, Valerie Fei Lee1; Aschtgen, Marie-Stephanie1,2,3,4; Qu, Jing5; Yang, Shuowei6; Xu, Guangfu6; Seng, Zi Jing2,19; Brown, Alexandra C.7,8; Ali, Md Khadem7,8,20; Jaggi, Tavleen K.1; Sankaran, Jagadish9,10,21; Foo, Yong Hwee2; Righetti, Francesco3,4; Nedumaran, Anu Maashaa11; Mac Aogain, Micheal1,22,23; Roizman, Dan2; Richard, Jean-Alexandre12; Rogers, Thomas R.13; Toyofuku, Masanori14; Luo, Dahai1; Loh, Edmund2,3,4; Wohland, Thorsten9,10; Czarny, Bertrand1,14; Horvat, Jay C.7,8; Hansbro, Philip M.7,8,15,16; Yang, Liang2,24; Li, Liang5; Normark, Staffan1,2,3,4; Normark, Birgitta Henriques1,2,3,4; Chotirmall, Sanjay H.1

2020-09-01

10.1128/mBio.01774-20

mBio   影响因子和分区

2150-7511

Estrogen, a major female sex steroid hormone, has been shown to promote the selection of mucoid Pseudomonas aeruginosa in the airways of patients with chronic respiratory diseases, including cystic fibrosis. This results in long-term persistence, poorer clinical outcomes, and limited therapeutic options. In this study, we demonstrate that at physiological concentrations, sex steroids, including testosterone and estriol, induce membrane stress responses in P. aeruginosa. This is characterized by increased virulence and consequent inflammation and release of proinflammatory outer membrane vesicles promoting in vivo persistence of the bacteria. The steroid-induced P. aeruginosa response correlates with the molecular polarity of the hormones and membrane fluidic properties of the bacteria. This novel mechanism of interaction between sex steroids and P. aeruginosa explicates the reported increased disease severity observed in females with cystic fibrosis and provides evidence for the therapeutic potential of the modulation of sex steroids to achieve better clinical outcomes in patients with hormone-responsive strains.

hormones steroids Pseudomonas aeruginosa membrane stress gender

SCI

英语

其他

Singapore Ministry of Health's National Medical Research Council[MOH-000141] ; National Health and Medical Research Council (NHMRC) of Australia[1175134] ; NIH[P30 DK089507] ; LKCMedicine[M4082195]

Microbiology

Microbiology

WOS:000579503600029

AMER SOC MICROBIOLOGY

Web of Science

1.Nanyang Technol Univ, Lee Kong Chian Sch Med, Singapore, Singapore
2.Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore, Singapore
3.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden
4.Karolinska Univ Hosp Solna, Stockholm, Sweden
5.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen, Guangdong, Peoples R China
6.Sun Yat Sen Univ, Dept Otolaryngol, Affiliated Hosp 5, Zhuhai, Guangdong, Peoples R China
7.Univ Newcastle, Prior Res Ctr Hlth Lungs, Hunter Med Res Inst, Newcastle, NSW, Australia
8.Univ Newcastle, Sch Biomed Sci & Pharm, Newcastle, NSW, Australia
9.Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore
10.Natl Univ Singapore, Ctr Bioimaging Sci, Singapore, Singapore
11.Nanyang Technol Univ, Sch Mat Sci & Engn MSE, Singapore, Singapore
12.ASTAR, Agcy Sci Technol & Res, Inst Chem & Engn Sci, Funct Mol & Polymers,ICES, Singapore, Singapore
13.Trinity Coll Dublin, Dept Clin Microbiol, St Jamess Hosp Campus, Dublin, Ireland
14.Univ Tsukuba, Fac Life & Environm Sci, Tsukuba, Ibaraki, Japan
15.Centenary Inst, Ctr Inflammat, Sydney, NSW, Australia
16.Univ Technol Sydney, Fac Sci, Sch Life Sci, Ultimo, NSW, Australia
17.Minist Hlth, Adm Sci Technol & Train, Hanoi, Vietnam
18.Univ Oxford, Oxford Univ, Clin Res Unit, Ho Chi Minh City, Vietnam
19.Bone Marrow Donor Programme, Singapore, Singapore
20.Stanford Univ, Sch Med, Div Pulm Allergy & Critical Care Med, Stanford, CA 94305 USA
21.Agcy Sci Technol & Res, ASTAR, Genome Inst Singapore, Singapore, Singapore
22.St James Hosp, Dept Biochem, Biochem Genet Lab, Dublin, Ireland
23.Trinity Coll Dublin, Sch Med, Dublin, Ireland
24.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China

Vidaillac, Celine,Yong, Valerie Fei Lee,Aschtgen, Marie-Stephanie,et al. Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa[J]. mBio,2020,11(5).

Vidaillac, Celine.,Yong, Valerie Fei Lee.,Aschtgen, Marie-Stephanie.,Qu, Jing.,Yang, Shuowei.,...&Chotirmall, Sanjay H..(2020).Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa.mBio,11(5).

Vidaillac, Celine,et al."Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa".mBio 11.5(2020).