Antimicrobial hydroxyapatite and its composites for the repair of infected femoral condyle

Tian，Xinggui1,2,3; Lu，Zhihui1; Ma，Chuying4; Wu，Min1; Zhang，Chengfei5; Yuan，Yuping6; Yuan，Xiaowei7; Xie，Denghui1; Liu，Chao4; Guo，Jinshan1

2021-02-01

10.1016/j.msec.2020.111807

Materials Science & Engineering C-Materials for Biological Applications   影响因子和分区

0928-4931

1873-0191

Orthopedic implant-associated infection constitutes one of the most devastating and challenging symptoms in the clinic. Implants without antimicrobial properties may become the harbourage for microbial colonization and biofilm formation, thus hindering normal bone regeneration processes. We had previously developed tannin modified HA (THA) as well as silver and tannin modified hydroxyapatite (HA) (Ag-THA) via a facile one-step and scalable process, and proven their antimicrobial performance in vitro. Herein, by compositing with non-antimicrobial polyurethane (PU), the in vivo anti-bacterial activity, osteoconductivity and osteoinductivity of PU/Ag-THA composite were investigated using an infected femoral condyle defect model on rat. PU/Ag-THA exhibited excellent in vivo antimicrobial activity, with the calculated bacteria fraction being reduced to lower than 3% at week 12 post operation. Meanwhile, PU/Ag-THA is also promising for bone regeneration under the bacteria challenge, evidenced by a final bone mineral density (BMD) ~0.6 times higher than that of the blank control at week 12. A continuous increase in BMD over time was observed in the PU/Ag-THA group, but not in the blank control and its non- or weak-antimicrobial counterparts (PU/HA and PU/THA), in which the growth rate of BMD declined after 8 weeks of operation. The enhanced osteoinductivity of PU/Ag-THA relative to blank control, PU/HA and PU/THA was also confirmed by the Runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN) immunohistochemical staining. The above findings suggest that antimicrobial Ag-THA may serve as a promising and easy-to-produce antimicrobial mineral for the development of antimicrobial orthopedic composite implants to address the challenges in orthopedic surgeries, especially where infection may become a challenging condition to treat.

Antimicrobial Bone regeneration Polyurethane Silver Tannin

EI ; SCI

英语

其他

WOS:000619120900006

20205209671456

Adhesives ; Flavonoids ; Polyurethanes ; Hydroxyapatite ; Bacteria ; Growth rate ; Transcription ; Tannins

Biology:461.9 ; Precious Metals:547.1 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Organic Polymers:815.1.1

2-s2.0-85097912549

Scopus

1.Department of Histology and Embryology,School of Basic Medical Sciences,Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases,The Third Affiliated Hospital of Southern Medical University,Southern Medical University,Guangzhou,China
2.Department of Orthopedics,The Affiliated Hospital of Southwest Medical University Luzhou,646000,China
3.University Hospital for Orthopedics and Accident Surgery (OUC),Carl Gustav Carus Dresden University Hospital,TU Dresden,Institute of Public Law of the Free State of Saxony,Dresden,Fetscherstrasse 74,01307,Germany
4.Aleo BME,Inc.,State College,200 Innovation Blvd, Suite 210A,16803,United States
5.Department of Dentistry,The University of Hong Kong,Hong Kong
6.Department of Material Science and Engineering,Southern University of Science and Technology,Shenzhen,518055,China
7.Department of Orthopedics,Shengjing Hospital of China Medical University,Shenyang,110004,China

Tian，Xinggui,Lu，Zhihui,Ma，Chuying,et al. Antimicrobial hydroxyapatite and its composites for the repair of infected femoral condyle[J]. Materials Science & Engineering C-Materials for Biological Applications,2021,121.

Tian，Xinggui.,Lu，Zhihui.,Ma，Chuying.,Wu，Min.,Zhang，Chengfei.,...&Guo，Jinshan.(2021).Antimicrobial hydroxyapatite and its composites for the repair of infected femoral condyle.Materials Science & Engineering C-Materials for Biological Applications,121.

Tian，Xinggui,et al."Antimicrobial hydroxyapatite and its composites for the repair of infected femoral condyle".Materials Science & Engineering C-Materials for Biological Applications 121(2021).