Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors

Zhang, Yali1,2; Wang, Shaojuan1,2; Wu, Yangtao1,2; Hou, Wangheng1,2; Yuan, Lunzhi1,2; Sheng, Chenguang3; Wang, Juan1,2; Ye, Jianghui1,2; Zheng, Qingbing1,2; Ma, Jian1,2; Xu, Jingjing4; Wei, Min1,2; Li, Zonglin1,2; Nian, Sheng1,2; Xiong, Hualong1,2; Zhang, Liang1,2; Shi, Yang1,2; Fu, Baorong1,2; Cao, Jiali1,2; Yang, Chuanlai1,2; Li, Zhiyong5; Yang, Ting4; Liu, Lei3; Yu, Hai1,2; Hu, Jianda4; Ge, Shengxiang1,2; Chen, Yixin1,2; Zhang, Tianying1,2; Zhang, Jun1,2; Cheng, Tong1,2; Yuan, Quan1,2; Xia, Ningshao1,2

2020-12-18

10.1002/smtd.202001031

Small Methods   影响因子和分区

2366-9608

The ongoing corona virus disease 2019 (COVID-19) pandemic, caused by SARS-CoV-2 infection, has resulted in hundreds of thousands of deaths. Cellular entry of SARS-CoV-2, which is mediated by the viral spike protein and ACE2 receptor, is an essential target for the development of vaccines, therapeutic antibodies, and drugs. Using a mammalian cell expression system, a genetically engineered sensor of fluorescent protein (Gamillus)-fused SARS-CoV-2 spike trimer (STG) to probe the viral entry process is developed. In ACE2-expressing cells, it is found that the STG probe has excellent performance in the live-cell visualization of receptor binding, cellular uptake, and intracellular trafficking of SARS-CoV-2 under virus-free conditions. The new system allows quantitative analyses of the inhibition potentials and detailed influence of COVID-19-convalescent human plasmas, neutralizing antibodies and compounds, providing a versatile tool for high-throughput screening and phenotypic characterization of SARS-CoV-2 entry inhibitors. This approach may also be adapted to develop a viral entry visualization system for other viruses.

entry inhibitor fluorescent protein high-content analysis live-cell imaging SARS-CoV-2 spike glycoprotein viral entry visualization

SCI ; EI

英语

其他

National Natural Science Foundation of China[81993149041][81902057][81871316][U1905205] ; National Science and Technology Major Project of Infectious Diseases[2017ZX10304402-002-003][2017ZX10202203-009] ; National Science and Technology Major Projects for Major New Drugs Innovation and Development[2018ZX09711003-005-003] ; Science and Technology Major Project of Fujian[2020YZ014001] ; Science and Technology Major Project of Xiamen[3502Z2020YJ01] ; Guangdong Basic and Applied Basic Research Foundation[2020A1515010368]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000599606500001

WILEY-V C H VERLAG GMBH

Web of Science

1.Xiamen Univ, Sch Publ Hlth, Natl Inst Diagnost & Vaccine Dev Infect Dis, State Key Lab Mol Vaccinol & Mol Diagnost, Xiamen 361102, Fujian, Peoples R China
2.Xiamen Univ, Sch Life Sci, Xiamen 361102, Fujian, Peoples R China
3.Southern Univ Sci & Technol, Hosp Affiliated 2, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis,Shenzhen Key Lab Pat, Shenzhen 518112, Guangdong, Peoples R China
4.Fujian Med Univ, Union Hosp, Fujian Prov Key Lab Hematol, Fujian Inst Hematol,Dept Hematol, Fuzhou 350001, Fujian, Peoples R China
5.Xiamen Univ, Hosp 1, Xiamen 361003, Peoples R China

Zhang, Yali,Wang, Shaojuan,Wu, Yangtao,et al. Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors[J]. Small Methods,2020,5(2).

Zhang, Yali.,Wang, Shaojuan.,Wu, Yangtao.,Hou, Wangheng.,Yuan, Lunzhi.,...&Xia, Ningshao.(2020).Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors.Small Methods,5(2).

Zhang, Yali,et al."Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors".Small Methods 5.2(2020).