Three-dimensional visualization and analysis of flowing droplets in microchannels using real-time quantitative phase microscopy

Luo，Yingdong1; Yang，Jinwu1; Zheng，Xinqi1; Wang，Jianjun1; Tu，Xin1; Che，Zhizhao2; Fang，Jiakun3; Xi，Lei4; Nguyen，Nam Trung5; Song，Chaolong1

2021-01-07

10.1039/d0lc00917b

LAB ON A CHIP   影响因子和分区

1473-0197

1473-0189

Recent years have witnessed the development of droplet-based microfluidics as a useful and effective tool for high-throughput analysis in biological, chemical and environmental sciences. Despite the flourishing development of droplet manipulation techniques, only a few methods allow for label-free and quantitative inspection of flowing droplets in microchannels in real-time and in three dimensions (3-D). In this work, we propose and demonstrate the application of a real-time quantitative phase microscopy (RT-QPM) technique for 3-D visualization of droplets, and also for full-field and label-free measurement of analyte concentration distribution in the droplets. The phase imaging system consists of a linear-CCD-based holographic microscopy configuration and an optofluidic phase-shifting element, which can be used for retrieving quantitative phase maps of flowing objects in the microchannels with a temporal resolution only limited to the frame rate of the CCD camera. To demonstrate the capabilities of the proposed imaging technique, we have experimentally validated the 3-D image reconstruction of the droplets generated in squeezing and dripping regimes and quantitatively investigated the volumetric and morphological variation of droplets as well as droplet parameters related to the depth direction under different flow conditions. We also demonstrated the feasibility of using this technique, as a refractive index sensor, for in-line quantitative measurement of carbamide analyte concentration within the flowing droplets.

SCI ; EI

英语

其他

National Natural Science Foundation of China[61804138,61905224,61775028,81571722] ; Wuhan Science and Technology Bureau[2020010601012164]

Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation

Biochemical Research Methods ; Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology ; Instruments & Instrumentation

WOS:000607297200015

ROYAL SOC CHEMISTRY

20210209745777

CCD cameras ; Chemical analysis ; Image reconstruction ; Image resolution ; Imaging techniques ; Microchannels ; Microfluidics ; Refractive index ; Refractometers ; Three dimensional computer graphics ; Visualization

Microfluidics:632.5.1 ; Semiconductor Devices and Integrated Circuits:714.2 ; Data Processing and Image Processing:723.2 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Optical Instruments:941.3

CHEMISTRY

2-s2.0-85098912262

Scopus

1.School of Mechanical Engineering and Electronic Information,China University of Geosciences,Wuhan,430074,China
2.State Key Laboratory of Engines,Tianjin University,Tianjin,300072,China
3.State Key Laboratory of Advanced Electromagnetic Engineering and Technology,School of Electrical and Electronic Engineering,Huazhong University of Science and Technology,Wuhan,China
4.Department of Biomedical Engineering,Southern University of Science and Technology,Shenzhen,China
5.Queensland Micro- and Nanotechnology Centre,Griffith University,Brisbane,170 Kessels Road,4111,Australia

Luo，Yingdong,Yang，Jinwu,Zheng，Xinqi,et al. Three-dimensional visualization and analysis of flowing droplets in microchannels using real-time quantitative phase microscopy[J]. LAB ON A CHIP,2021,21(1):75-82.

Luo，Yingdong.,Yang，Jinwu.,Zheng，Xinqi.,Wang，Jianjun.,Tu，Xin.,...&Song，Chaolong.(2021).Three-dimensional visualization and analysis of flowing droplets in microchannels using real-time quantitative phase microscopy.LAB ON A CHIP,21(1),75-82.

Luo，Yingdong,et al."Three-dimensional visualization and analysis of flowing droplets in microchannels using real-time quantitative phase microscopy".LAB ON A CHIP 21.1(2021):75-82.