Expression and prognostic role of PLOD1 in malignant glioma

Wang，Hao; Luo，Weijian; Dai，Limeng

2020

10.2147/OTT.S265866

OncoTargets and Therapy   影响因子和分区

1178-6930

1178-6930

Background: Malignant glioma is rarely curable, and factors that influence the prognosis of glioma patients are not fully understood. Lysyl hydroxylases such as PLOD1 promote the cross-linking in extracellular matrix (ECM) molecules, which contribute to ECM structural stability and maturation. However, the expression and prognostic role of PLOD1 in malig- nant glioma remained to be determined. Methods: The expression of PLOD1 was evaluated by immunohistochemistry in 72 malig- nant glioma patients from Shenzhen People’s hospital. The mRNA expression profiles and clinical information of malignant glioma patients were obtained from public databases, including TCGA, CGGA, Rembrandt, and Gravendeel. The correlation between gene expres- sion and tumor grade, and IDH1/2 status and 1p19q status were evaluated. The association between gene expression and overall survival of malignant glioma patients was examined using Kaplan-Meier survival analysis. GO and KEGG pathways were analyzed by Metascape. Transwell invasion assays were performed to determine the effect of PLOD1 on migration and invasion of glioma cells in vitro. Results: PLOD1 expression was significantly elevated in malignant glioma tissues com- pared with non-tumor brain tissues. Besides, elevated levels of PLOD1 were significantly correlated with high tumor grade, wildtype IDH1/2 status, and 1p19q non-codel in all the four public datasets and in-house cohort. Malignant glioma patients with high PLOD1 expression had better overall survival compared to those with low PLOD1 expression. More importantly, patients with IDH1/2 mutations, 1p19q codeletions, and PLOD1 over- expression had the best overall survival. GO enrichment pathway analysis indicated that PLOD1 participates in regulating the extracellular matrix. Transwell invasion assay, which revealed that inhibiting PLOD1 reduced cell invasion in both U87 and U251 cells. Conclusion: PLOD1 serves as a potential prognostic marker and therapeutic target for malignant glioma.

Biomarker Glioma Invasion PLOD1 Prognosis

SCI

英语

第一 ; 通讯

WOS:000603399000005

2-s2.0-85098970017

Scopus

Department of Neurosurgery,Shenzhen People’s Hospital (Second Clinical Medical College),Jiʼnan University,The First Affiliated Hospital,Southern University of Science and Technology,Shenzhen,China

Wang，Hao,Luo，Weijian,Dai，Limeng. Expression and prognostic role of PLOD1 in malignant glioma[J]. OncoTargets and Therapy,2020,13:13285-13297.

Wang，Hao,Luo，Weijian,&Dai，Limeng.(2020).Expression and prognostic role of PLOD1 in malignant glioma.OncoTargets and Therapy,13,13285-13297.

Wang，Hao,et al."Expression and prognostic role of PLOD1 in malignant glioma".OncoTargets and Therapy 13(2020):13285-13297.