南方科技大学知识苑(SUSTech KC): Effects of Zoledronic Acid on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via Signal Transducer and Activator o f Transcription 3 Signaling Pathway

题名	Effects of Zoledronic Acid on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via Signal Transducer and Activator o f Transcription 3 Signaling Pathway
作者	Wan, Jun ; Ding, Guanggui ; Ling, Xiean ; Zhou, Min ; Wang, Jian 1
通讯作者	Wang, Jian
发表日期	2020-11-01
DOI	10.1166/jbt.2020.2487
发表期刊	Journal of Biomaterials and Tissue Engineering 影响因子和分区
ISSN	2157-9083
EISSN	2157-9091
卷号	10 期号:11
摘要	Zoledronic acid is an antitumor drug. STAT3 signaling pathway is positively correlated with non-small cell lung cancer (NSCLC). However, whether zoledronic acid affects the proliferation and apoptosis of NSCLC through STAT3 signaling pathway is unclear. The control group (NC group), zoledronic acid group (ZOLEDA group), and zoledronic acid + epidermal growth factor EGF group (ZOLEDA + EGF group) were set up followed by analysis of STAT3 mRNA level by qRT-PCR; p-STAT3, STAT3, Cyclin D1, Bcl-2, and Tubulin protein expression by Western blot, cell proliferation by clone formation and MTT assay, and cell apoptosis by Annexin V-FITC/PI staining. Compared with NC group, ZOLEDA group showed significantly reduced p-STAT3/STAT3 ratio and expression of Cyclin D1, and Bcl-2 protein (P < 0.01), which were significantly increased in ZOLEDA + EGF group (P < 0.05); clone formation and MTT results showed that compared with NC group, cell proliferation in ZOLEDA group was significantly reduced (P < 0.01), and increased in ZOLEDA + EGF group (P < 0.05). In addition, ZOLEDA group presented significantly promoted cell apoptosis (P < 0.01), which was decreased in ZOLEDA + EGF group (P < 0.05). Zoledronic acid (ZOLEDA) can inhibit STAT3 signaling pathway and cell proliferation and promote cell apoptosis. STAT3 signaling pathway activator EGF can prevent the effect of zoledronic acid.
关键词	Zoledronic Acid STAT3 Signaling Pathway Non-Small Cell Lung Cancer Cell Proliferation Apoptosis
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	Natural Science Foundation of Guangdong Province[2018A0303130247]
WOS研究方向	Cell Biology
WOS类目	Cell & Tissue Engineering
WOS记录号	WOS:000608978900009
出版者	AMER SCIENTIFIC PUBLISHERS
来源库	Web of Science
引用统计	被引频次[WOS]：0
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221086
专题	南方科技大学第一附属医院
作者单位	1.Jinan Univ, Clin Med Coll 2, Dept Thorac Surg, Shenzhen Peoples Hosp, Shenzhen 518020, Guangdong, Peoples R China 2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Wan, Jun,Ding, Guanggui,Ling, Xiean,et al. Effects of Zoledronic Acid on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via Signal Transducer and Activator o f Transcription 3 Signaling Pathway[J]. Journal of Biomaterials and Tissue Engineering,2020,10(11).
APA	Wan, Jun,Ding, Guanggui,Ling, Xiean,Zhou, Min,&Wang, Jian.(2020).Effects of Zoledronic Acid on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via Signal Transducer and Activator o f Transcription 3 Signaling Pathway.Journal of Biomaterials and Tissue Engineering,10(11).
MLA	Wan, Jun,et al."Effects of Zoledronic Acid on Proliferation and Apoptosis of Non-Small Cell Lung Cancer Cells via Signal Transducer and Activator o f Transcription 3 Signaling Pathway".Journal of Biomaterials and Tissue Engineering 10.11(2020).