MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer

Xu, Zhenglei1; Zhang, Dingguo1; Zhang, Zhuliang1; Luo, Weixiang2; Shi, Ruiyue1; Yao, Jun1; Li, Defeng1; Wang, Lisheng1; Liao, Bihong3

2021-01-15

10.3389/fcell.2020.625056

Frontiers in Cell and Developmental Biology   影响因子和分区

2296-634X

["Background: MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play vital regulatory roles in pancreatic cancer (PC) initiation and progression. We aimed to explore the biological functions and underlying mechanisms of miR-505-3p (miR-505) in PC.","Methods: We first screened miRNA expression profiles using microarray in PC tissues and normal tissues, and then studied the function and underlying mechanism of miR-505. Moreover, we evaluated the regulatory effect of lncRNA LINC01448 on miR-505.","Results: We demonstrated miR-505 that was significantly downregulated in PC tissues. We further revealed that miR-505 significantly inhibited cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by targeting HK2. In addition, overexpression of miR-505 led to tumor growth inhibition in vivo, demonstrating that it acts as a tumor suppressor in PC. LINC01448 was identified as an oncogenic lncRNA that could reduce miR-505 expression. Subsequent studies confirmed that LINC01448 enhanced cell proliferation, invasion, sphere formation, glucose consumption, and lactate production by regulating the miR-505/HK2 pathway.","Conclusions: These findings demonstrated that miR-505, suppressed by LINC01448, could function as a key tumor suppressor by targeting HK2 in PC, elucidating an important role of the LINC01448/miR-505/HK2 pathway in regulating PC glycolysis and progression."]

long non-coding RNA microRNA miR-505 HK2 pancreatic cancer

SCI

英语

通讯

Natural Science Foundation of Guangdong Province[2018A0303130278]

Cell Biology ; Developmental Biology

Cell Biology ; Developmental Biology

WOS:000612656300001

FRONTIERS MEDIA SA

Web of Science

1.Jinan Univ, Shenzhen Peoples Hosp, Dept Gastroenterol, Clin Med Coll 2, Shenzhen, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Jinan Univ,Nursing Dept,Affiliated Clin Med Coll, Shenzhen, Peoples R China
3.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Jinan Univ,Dept Cardiol,Affiliated Clin Med Coll, Shenzhen, Peoples R China

Xu, Zhenglei,Zhang, Dingguo,Zhang, Zhuliang,et al. MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer[J]. Frontiers in Cell and Developmental Biology,2021,8.

Xu, Zhenglei.,Zhang, Dingguo.,Zhang, Zhuliang.,Luo, Weixiang.,Shi, Ruiyue.,...&Liao, Bihong.(2021).MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer.Frontiers in Cell and Developmental Biology,8.

Xu, Zhenglei,et al."MicroRNA-505, Suppressed by Oncogenic Long Non-coding RNA LINC01448, Acts as a Novel Suppressor of Glycolysis and Tumor Progression Through Inhibiting HK2 Expression in Pancreatic Cancer".Frontiers in Cell and Developmental Biology 8(2021).