Improving the Accuracy of Pdot-Based Continuous Glucose Monitoring by Using External Ratiometric Calibration

Sun, Kai1; Liu, Siyang2; Liu, Jing2; Ding, Zhaoyang1; Jiang, Yifei1; Zhang, Jicheng1; Chen, Haobin1; Yu, Jiangbo1; Wu, Changfeng2; Chiu, Daniel T.1

2021-02-02

10.1021/acs.analchem.0c04223

ANALYTICAL CHEMISTRY   影响因子和分区

0003-2700

1520-6882

Continuous glucose monitoring (CGM) allows type I and II diabetes patients to track changes in their glucose levels, allowing detection of impending hypoglycemia or hyperglycemia. Polymer dots (Pdots) are candidates for use in implanted CGM systems due to their exceptional brightness, photostability, sensitivity, and biocompatibility. However, Pdot glucose transducers are oxygen-dependent, and changes in tissue oxygen levels affect their measurement accuracy. Here, we describe an external ratiometric calibration method that corrects for changes in tissue oxygen levels to improve measurement accuracy. This method uses the ratio of oxygen concentrations inside and outside the Pdot glucose transducer as an indicator of glucose concentration to correct for signal deviations caused by tissue oxygen fluctuations. A second oxygen-sensitive Pdot that is not conjugated with glucose oxidase is used to measure the oxygen concentration outside the Pdot glucose transducer. We describe the theoretical basis for this approach and demonstrate its effectiveness experimentally in a subcutaneous mouse implant model. This external ratiometric system achieves higher accuracy glucose measurements than previous Pdot-based CGM systems and comparable accuracy to current commercial CGM systems, demonstrating the utility of the external ratiometric calibration strategy.

SCI ; EI

英语

NI论文

通讯

National Institutes of Health[R01MH113333] ; Shenzhen Science and Technology Innovation Commission[KQTD20170810111314625] ; National Natural Science Foundation of China[81771930] ; National Key Research and Development Program of China[2018YFB0407200]

Chemistry

Chemistry, Analytical

WOS:000618089100059

AMER CHEMICAL SOC

20210509858512

Biocompatibility ; Calibration ; Glucose ; Glucose sensors ; Oxygen ; Tissue ; Transducers

Bioengineering and Biology:461 ; Biomedical Equipment, General:462.1 ; Chemical Products Generally:804 ; Organic Compounds:804.1

CHEMISTRY

Web of Science

1.Univ Washington, Dept Chem & Bioengn, Seattle, WA 98195 USA
2.Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 518055, Guangdong, Peoples R China

Sun, Kai,Liu, Siyang,Liu, Jing,et al. Improving the Accuracy of Pdot-Based Continuous Glucose Monitoring by Using External Ratiometric Calibration[J]. ANALYTICAL CHEMISTRY,2021,93(4):2359-2366.

Sun, Kai.,Liu, Siyang.,Liu, Jing.,Ding, Zhaoyang.,Jiang, Yifei.,...&Chiu, Daniel T..(2021).Improving the Accuracy of Pdot-Based Continuous Glucose Monitoring by Using External Ratiometric Calibration.ANALYTICAL CHEMISTRY,93(4),2359-2366.

Sun, Kai,et al."Improving the Accuracy of Pdot-Based Continuous Glucose Monitoring by Using External Ratiometric Calibration".ANALYTICAL CHEMISTRY 93.4(2021):2359-2366.