南方科技大学知识苑(SUSTech KC): CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY

题名	CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY
作者	Xie, Hongliang; Tang, Jianming; Lu, Lu; Li, Bohan; Wang, Mengmeng
通讯作者	Xie, Hongliang
发表日期	2021
DOI	10.1111/odi.13759
发表期刊	ORAL DISEASES 影响因子和分区
ISSN	1354-523X
EISSN	1601-0825
摘要	["Objective The study was designed to explore the role of cancer susceptibility candidate 9 (CASC9) in salivary adenoid cystic carcinoma (SACC) (SACC-83 and SACC-LM) cell malignant phenotypes.","Methods Colony formation assay was used to measure cell proliferation. Transwell assay was used to detect cell migration and invasion. Flow cytometry analysis was applied to determine cell cycle distribution and apoptosis. FISH assay revealed the subcellular location of CASC9.","Results Downregulation of CASC9 inhibited SACC cell proliferation, migration, and invasion, led to cell arrest at G0/G1 phase, and facilitated cell apoptosis. In mechanism, CASC9 bound with microRNA 146b-5p (miR-146b-5p) and negatively modulated miR-146b-5p expression. MiR-146b-5p directly targeted 3' untranslated region of ATP-Citrate Lyase (ACLY) to degrade ACLY in SACC cells. CASC9 upregulated ACLY expression through competitively binding with miR-146b-5p. Furthermore, rescue assays indicated that ACLY overexpression counteracted the effects triggered by CASC9 knockdown on cell proliferation, migration, invasion, and apoptosis in SACC cells.","Conclusion CASC9 facilitated the malignant phenotypes of SACC cells by the regulation of the miR-146b-5p/ACLY axis. These findings might lay foundation for SACC research."]
关键词	ACLY CASC9 miR‐ 146b‐ 5p salivary adenoid cystic carcinoma
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	第一 ; 通讯
WOS研究方向	Dentistry, Oral Surgery & Medicine
WOS类目	Dentistry, Oral Surgery & Medicine
WOS记录号	WOS:000604664500001
出版者	WILEY
ESI学科分类	CLINICAL MEDICINE
来源库	Web of Science
引用统计	被引频次[WOS]：7
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221110
专题	南方科技大学第一附属医院
作者单位	Jinan Univ, South Univ Sci & Technol, Clin Med Coll 2,Dept Oral & Maxillofacial Surg, Affiliated Hosp 1,Shenzhen Peoples Hosp,Stomatol, Shenzhen, Peoples R China
第一作者单位	南方科技大学第一附属医院
通讯作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Xie, Hongliang,Tang, Jianming,Lu, Lu,et al. CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY[J]. ORAL DISEASES,2021.
APA	Xie, Hongliang,Tang, Jianming,Lu, Lu,Li, Bohan,&Wang, Mengmeng.(2021).CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY.ORAL DISEASES.
MLA	Xie, Hongliang,et al."CASC9 plays a role in salivary adenoid cystic carcinoma in vitro by upregulation of ACLY".ORAL DISEASES (2021).