Quantitatively Monitoring In Situ Mitochondrial Thermal Dynamics by Upconversion Nanoparticles

Di, Xiangjun1; Wang, Dejiang1; Zhou, Jiajia1; Zhang, Lin2; Stenzel, Martina H.2; Su, Qian Peter1,3; Jin, Dayong1,4

2021-02-24

10.1021/acs.nanolett.0c04281

NANO LETTERS   影响因子和分区

1530-6984

1530-6992

Temperature dynamics reflect the physiological conditions of cells and organisms. Mitochondria regulate the temperature dynamics in living cells as they oxidize the respiratory substrates and synthesize ATP, with heat being released as a byproduct of active metabolism. Here, we report an upconversion nanoparticle-based thermometer that allows the in situ thermal dynamics monitoring of mitochondria in living cells. We demonstrate that the upconversion nanothermometers can efficiently target mitochondria, and the temperature-responsive feature is independent of probe concentration and medium conditions. The relative sensing sensitivity of 3.2% K-1 in HeLa cells allows us to measure the mitochondrial temperature difference through the stimulations of high glucose, lipid, Ca2+ shock, and the inhibitor of oxidative phosphorylation. Moreover, cells display distinct response time and thermodynamic profiles under different stimulations, which highlight the potential applications of this thermometer to study in situ vital processes related to mitochondrial metabolism pathways and interactions between organelles.

Upconversion Nanoparticles (UCNPs) Biosensing Nanothermometry Mitochondria

SCI ; EI

英语

NI论文

通讯

Australia National Health and Medical Council (NHMRC)[APP1177374] ; Australia National Heart Foundation (NHF)[102592] ; Australian Research Council Discovery Early Career Researcher Award Scheme[DE180100669] ; National Natural Science Foundation of China (NSFC)[61729501] ; Major International (Regional) Joint Research Project of NSFC[51720105015] ; Science and Technology Innovation Commission of Shenzhen[KQTD20170810110913065] ; Australia China Science and Research Fund Joint Research Centre for Point-of-Care Testing["ACSRF658277","SQ2017YFGH001190"] ; China Scholarship Council[201706170028,201706170027]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000624334100013

AMER CHEMICAL SOC

20211010027657

Atmospheric thermodynamics ; Cytology ; Dynamics ; Lanthanum compounds ; Metabolism ; Nanoparticles ; Potassium compounds ; Temperature ; Thermometers

Biological Materials and Tissue Engineering:461.2 ; Thermodynamics:641.1 ; Nanotechnology:761 ; Solid State Physics:933 ; Temperature Measuring Instruments:944.5

MATERIALS SCIENCE

Web of Science

1.Univ Technol Sydney, Fac Sci, Inst Biomed Mat & Devices IBMD, Sydney, NSW 2007, Australia
2.Univ New South Wales, Sch Chem, Cluster Adv Macromol Design, Sydney, NSW 2052, Australia
3.Univ Technol Sydney, Fac Engn & Informat Technol, Sch Biomed Engn, Sydney, NSW 2007, Australia
4.Southern Univ Sci & Technol, UTS SUStech Joint Res Ctr Biomed Mat & Devices, Dept Biomed Engn, Shenzhen 518055, Peoples R China

Di, Xiangjun,Wang, Dejiang,Zhou, Jiajia,et al. Quantitatively Monitoring In Situ Mitochondrial Thermal Dynamics by Upconversion Nanoparticles[J]. NANO LETTERS,2021,21(4):1651-1658.

Di, Xiangjun.,Wang, Dejiang.,Zhou, Jiajia.,Zhang, Lin.,Stenzel, Martina H..,...&Jin, Dayong.(2021).Quantitatively Monitoring In Situ Mitochondrial Thermal Dynamics by Upconversion Nanoparticles.NANO LETTERS,21(4),1651-1658.

Di, Xiangjun,et al."Quantitatively Monitoring In Situ Mitochondrial Thermal Dynamics by Upconversion Nanoparticles".NANO LETTERS 21.4(2021):1651-1658.