Genetic Structure, Function, and Evolution of Capsule Biosynthesis Loci in Vibrio parahaemolyticus

Bian, Shengzhe1,2,3,7; Zeng, Wenhong4; Li, Qiwen2,3; Li, Yinghui5; Wong, Nai-Kei6; Jiang, Min5; Zuo, Le5; Hu, Qinghua5; Li, Liqiang2,3

2021-01-11

10.3389/fmicb.2020.546150

Frontiers in Microbiology   影响因子和分区

1664-302X

Capsule-forming extracellular polysaccharides are crucial for bacterial host colonization, invasion, immune evasion, and ultimately pathogenicity. Due to warming ocean waters and human encroachment of coastal ecosystems, Vibrio parahaemolyticus has emerged as a globally important foodborne enteropathogen implicated in acute gastroenteritis, wound infections, and septic shock. Conventionally, the antigenic properties of lipopolysaccharide (LPS, O antigen) and capsular polysaccharide (CPS, K antigen) have provided a basis for serotyping V. parahaemolyticus, whereas disclosure of genetic elements encoding 13 O-serogroups have allowed molecular serotyping methods to be developed. However, the genetic structure of CPS loci for 71 K-serogroups has remained unidentified, limiting progress in understanding its roles in V. parahaemolyticus pathophysiology. In this study, we identified and characterized the genetic structure and their evolutionary relationship of CPS loci of 40 K-serogroups through whole genome sequencing of 443 V. parahaemolyticus strains. We found a distinct pattern of CPS gene cluster across different K-serogroups and expanded its new 3 '-border by identifying glpX as a key gene conserved across all K-serogroups. A total of 217 genes involved in CPS biosynthesis were annotated. Functional contents and genetic structure of the 40 K-serogroups were analyzed. Based on inferences from species trees and gene trees, we proposed an evolution model of the CPS gene clusters of 40 K-serogroups. Horizontal gene transfer by recombination from other Vibrio species, gene duplication is likely to play instrumental roles in the evolution of CPS in V. parahaemolyticus. This is the first time, to the best of our knowledge, that a large scale of CPS gene clusters of different K-serogroups in V. parahaemolyticus have been identified and characterized in evolutionary contexts. This work should help advance understanding on the variation of CPS in V. parahaemolyticus and provide a framework for developing diagnostically relevant serotyping methods.

genetic structure K antigen capsule biosynthesis loci Vibiro parahaemolyticus serotype

SCI

英语

其他

National Science and Technology Major Project of China[2017ZX10303406] ; National Natural Science Foundation of China[81773436] ; Sanming Project of Medicine in Shenzhen[SZSM201811071]

Microbiology

Microbiology

WOS:000611004100001

FRONTIERS MEDIA SA

Web of Science

1.Univ Chinese Acad Sci, BGI Educ Ctr, Shenzhen, Peoples R China
2.BGI Shenzhen, Shenzhen, Peoples R China
3.Shenzhen Key Lab Unknown Pathogen Identificat, Shenzhen, Peoples R China
4.Jiangxi Univ Tradit Chinese Med, Nanchang, Jiangxi, Peoples R China
5.Shenzhen Ctr Dis Control & Prevent, Shenzhen, Peoples R China
6.Southern Univ Sci & Technol, Hosp Affiliated 2, Shenzhen Peoples Hosp 3, Natl Clin Res Ctr Infect Dis, Shenzhen, Peoples R China
7.Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Guangzhou, Peoples R China

Bian, Shengzhe,Zeng, Wenhong,Li, Qiwen,et al. Genetic Structure, Function, and Evolution of Capsule Biosynthesis Loci in Vibrio parahaemolyticus[J]. Frontiers in Microbiology,2021,11.

Bian, Shengzhe.,Zeng, Wenhong.,Li, Qiwen.,Li, Yinghui.,Wong, Nai-Kei.,...&Li, Liqiang.(2021).Genetic Structure, Function, and Evolution of Capsule Biosynthesis Loci in Vibrio parahaemolyticus.Frontiers in Microbiology,11.

Bian, Shengzhe,et al."Genetic Structure, Function, and Evolution of Capsule Biosynthesis Loci in Vibrio parahaemolyticus".Frontiers in Microbiology 11(2021).