The Pyocin Regulator PrtR Regulates Virulence Expression of Pseudomonas aeruginosa by Modulation of Gac/Rsm System and c-di-GMP Signaling Pathway

Jiao, Hongying1; Li, Fan2; Wang, Tietao1; Yam, Joey Kuok Hoong3; Yang, Liang4; Liang, Haihua1; Whiteley, Marvin5

2021-02-01

10.1128/IAI.00602-20

INFECTION AND IMMUNITY   影响因子和分区

0019-9567

1098-5522

In Pseudomonas aeruginosa, the second messenger cyclic-di-GMP and Gac/Rsm signaling pathways are associated with the transition from acute to chronic infection. Therefore, identification of the molecular mechanisms that govern lifestyle choice in bacteria is very important. Here, we identified a novel cyclic-di-GMP modulator, PrtR, which was shown to repress pyocin production by inhibition of PrtN and activate the type III secretion system (T3SS) through PtrB. Compared to a wild-type strain or a prtN mutant, the prtR prtN double mutant exhibited a wrinkly colony and hyperbiofilm phenotype, as well as an increase in intracellular c-di-GMP levels. Interestingly, a diguanylate cyclase (DGC) gene, siaD, was repressed by PrtR. Further experiments revealed that PrtR directly interacts with SiaD and facilitates the accumulation of c-di-GMP in cells. We also demonstrated that PrtR regulates the activity of the Gac/Rsm system, thus affecting expression of the T3SS and type VI secretion system (T6SS) and the formation of biofilm. Taken together, the present findings indicate that PrtR, as a c-di-GMP modulator, plays key roles in the adaptation to opportunistic infection of P. aeruginosa. Additionally, this study revealed a novel mechanism for PrtR-mediated regulation of the lifestyle transition via the Gac/Rsm and c-di-GMP signaling networks.

cyclic di-GMP pyocin regulator PrtR motile-sessile switch Gac/Rsm system Pseudomonas aeruginosa

SCI

英语

其他

Immunology ; Infectious Diseases

Immunology ; Infectious Diseases

WOS:000609500500012

AMER SOC MICROBIOLOGY

IMMUNOLOGY

Web of Science

1.Northwest Univ, Coll Life Sci, Key Lab Resources Biol & Biotechnol Western China, Minist Educ, Xian, Shaanxi, Peoples R China
2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling, Shaanxi, Peoples R China
3.Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Sch Biol Sci, Singapore, Singapore
4.Southern Univ Sci & Technol, Sch Med, Shenzhen, Peoples R China
5.Georgia Inst Technol, Sch Biol Sci, Atlanta, GA 30332 USA

Jiao, Hongying,Li, Fan,Wang, Tietao,et al. The Pyocin Regulator PrtR Regulates Virulence Expression of Pseudomonas aeruginosa by Modulation of Gac/Rsm System and c-di-GMP Signaling Pathway[J]. INFECTION AND IMMUNITY,2021,89(2).

Jiao, Hongying.,Li, Fan.,Wang, Tietao.,Yam, Joey Kuok Hoong.,Yang, Liang.,...&Whiteley, Marvin.(2021).The Pyocin Regulator PrtR Regulates Virulence Expression of Pseudomonas aeruginosa by Modulation of Gac/Rsm System and c-di-GMP Signaling Pathway.INFECTION AND IMMUNITY,89(2).

Jiao, Hongying,et al."The Pyocin Regulator PrtR Regulates Virulence Expression of Pseudomonas aeruginosa by Modulation of Gac/Rsm System and c-di-GMP Signaling Pathway".INFECTION AND IMMUNITY 89.2(2021).