南方科技大学知识苑(SUSTech KC): Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling

题名	Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling
作者	Zhuang, Changshui 1; Liu, Ying 2 ; Fu, Shengqiang 3; Yuan, Chaobo 4; Luo, Jingwen 5; Huang, Xueting 6; Yang, Weifeng 1; Xie, Wuwei 7; Zhuang, Chengle 7
通讯作者	Xie, Wuwei; Zhuang, Chengle
发表日期	2020-12-09
DOI	10.3389/fmolb.2020.616768
发表期刊	FRONTIERS IN MOLECULAR BIOSCIENCES 影响因子和分区
EISSN	2296-889X
卷号	7
摘要	A subset of long non-coding RNAs (lncRNAs), categorized as miRNA-host gene lncRNAs (lnc-miRHGs), is processed to produce miRNAs and involved in cancer progression. This work aimed to investigate the influences and the molecular mechanisms of lnc-miRHGs MIR497HG in bladder cancer (BCa). The miR-497 and miR-195 were derived from MIR497HG. We identified that lnc-miRHG MIR497HG and two harbored miRNAs, miR-497 and miR-195, were downregulated in BCa by analyzing The Cancer Genome Atlas and our dataset. Silencing of MIR497HG by CRISPR/Cas13d in BCa cell line 5637 promoted cell growth, migration, and invasion in vitro. Conversely, overexpression of MIR497HG suppressed cell progression in BCa cell line T24. MiR-497/miR-195 mimics rescued significantly the oncogenic roles of knockdown of MIR497HG by CRISPR/Cas13d in BCa. Mechanistically, miR-497 and miR-195 co-ordinately suppressed multiple key components in Hippo/Yap and transforming growth factor beta signaling and particularly attenuated the interaction between Yap and Smad3. In addition, E2F4 was proven to be critical for silencing MIR497HG transcription in BCa cells. In short, we propose for the first time to reveal the function and mechanisms of MIR497HG in BCa. Blocking the pathological process may be a potential strategy for the treatment of BCa.
关键词	bladder cancer MIR497HG CRISPR Cas13d Yap TGF-β
相关链接	[来源记录]
收录类别	SCI
语种	英语
学校署名	其他
资助项目	"San-ming" Project of Medicine in Shenzhen[SZSM201612066]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000600956600001
出版者	FRONTIERS MEDIA SA
来源库	Web of Science
引用统计	被引频次[WOS]：27
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221216
专题	南方科技大学第一附属医院
作者单位	1.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Dept Urol, Shenzhen, Peoples R China 2.Jinan Univ, Shenzhen Peoples Hosp, Affiliated Hosp 1, Southern Univ Sci & Technol,Second Clin Med Coll, Shenzhen, Peoples R China 3.Nanchang Univ, Dept Urol, Affiliated Hosp 1, Nanchang, Jiangxi, Peoples R China 4.Huazhong Univ Sci & Technol, Union Shenzhen Hosp, Emergency Dept, Shenzhen, Peoples R China 5.Shenzhen Yantian Dist Peoples Hosp, Shenzhen, Peoples R China 6.Huazhong Univ Sci & Technol, Dept Thorac Surg, Union Shenzhen Hosp, Shenzhen, Peoples R China 7.Peking Univ, Dept Urol, Shenzhen Hosp, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Zhuang, Changshui,Liu, Ying,Fu, Shengqiang,et al. Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling[J]. FRONTIERS IN MOLECULAR BIOSCIENCES,2020,7.
APA	Zhuang, Changshui.,Liu, Ying.,Fu, Shengqiang.,Yuan, Chaobo.,Luo, Jingwen.,...&Zhuang, Chengle.(2020).Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling.FRONTIERS IN MOLECULAR BIOSCIENCES,7.
MLA	Zhuang, Changshui,et al."Silencing of lncRNA MIR497HG via CRISPR/Cas13d Induces Bladder Cancer Progression Through Promoting the Crosstalk Between Hippo/Yap and TGF-beta/Smad Signaling".FRONTIERS IN MOLECULAR BIOSCIENCES 7(2020).