Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus

Huang, Guoqiang1,2; Xiao, Yanan3,4; Pi, Xiong1,2; Zhao, Liang1,2; Zhu, Qingjun3,4; Wang, Wenda3; Kuang, Tingyun3; Han, Guangye3; Sui, Sen-Fang1,2,5; Shen, Jian-Ren3,6,7

2021-02-02

10.1073/pnas.2018053118

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   影响因子和分区

0027-8424

Photosystem II (PSII) is a multisubunit pigment-protein complex and catalyzes light-driven water oxidation, leading to the conversion of light energy into chemical energy and the release of molecular oxygen. Psb27 is a small thylakoid lumen-localized protein known to serve as an assembly factor for the biogenesis and repair of the PSII complex. The exact location and binding fashion of Psb27 in the intermediate PSII remain elusive. Here, we report the structure of a dimeric Psb27-PSII complex purified from a psbV deletion mutant (Delta PsbV) of the cyanobacterium Thermosynechococcus vulcanus, solved by cryo-electron microscopy. Our structure showed that Psb27 is associated with CP43 at the luminal side, with specific interactions formed between Helix 2 and Helix 3 of Psb27 and a loop region between Helix 3 and Helix 4 of CP43 (loop C) as well as the large, lumen-exposed and hydrophilic E-loop of CP43. The binding of Psb27 imposes some conflicts with the N-terminal region of PsbO and also induces some conformational changes in CP43, CP47, and D2. This makes PsbO unable to bind in the Psb27-PSII. Conformational changes also occurred in D1, PsbE, PsbF, and PsbZ; this, together with the conformational changes occurred in CP43, CP47, and D2, may prevent the binding of PsbU and induce dissociation of PsbJ. This structural information provides important insights into the regulation mechanism of Psb27 in the biogenesis and repair of PSII.

photosystem II cryo-EM assembly repair Psb27

SCI

英语

NI期刊

通讯

National Key R&D Program of China["2017YFA0503700","2016YFA0501101","2017YFA0504600","2019YFA0906300"] ; National Natural Science Foundation of China[31470339] ; Strategic Priority Research Program of Chinese Academy of Science (CAS)[XDB17000000] ; CAS Key Research program for Frontier Science[QYZDY-SSW-SMC003] ; Youth Innovation Promotion Association of CAS[2020081]

Science & Technology - Other Topics

Multidisciplinary Sciences

WOS:000615345200035

NATL ACAD SCIENCES

BIOLOGY & BIOCHEMISTRY;CLINICAL MEDICINE;MULTIDISCIPLINARY;PLANT & ANIMAL SCIENCE;ENVIRONMENT/ECOLOGY;SOCIAL SCIENCES, GENERAL;MICROBIOLOGY;ECONOMICS BUSINESS;IMMUNOLOGY;MATERIALS SCIENCE;MATHEMATICS;SPACE SCIENCE;MOLECULAR BIOLOGY & GENETICS;PHARMACOLOGY & TOXICOLOGY;CHEMISTRY;PSYCHIATRY/PSYCHOLOGY;NEUROSCIENCE & BEHAVIOR;PHYSICS;GEOSCIENCES;AGRICULTURAL SCIENCES;ENGINEERING

Web of Science

1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Sch Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China
2.Tsinghua Univ, Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing 100084, Peoples R China
3.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Southern Univ Sci & Technol, Dept Biol, Shenzhen 518055, Guangdong, Peoples R China
6.Okayama Univ, Res Inst Interdisciplinary Sci, Okayama 7008530, Japan
7.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan

Huang, Guoqiang,Xiao, Yanan,Pi, Xiong,et al. Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118(5).

Huang, Guoqiang.,Xiao, Yanan.,Pi, Xiong.,Zhao, Liang.,Zhu, Qingjun.,...&Shen, Jian-Ren.(2021).Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118(5).

Huang, Guoqiang,et al."Structural insights into a dimeric Psb27-photosystem II complex from a cyanobacterium Thermosynechococcus vulcanus".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118.5(2021).