Amynthas corticis genome reveals molecular mechanisms behind global distribution

Wang, Xing1,2; Zhang, Yi3; Zhang, Yufeng4; Kang, Mingming5; Li, Yuanbo1,2; James, Samuel W.6; Yang, Yang7; Bi, Yanmeng8; Jiang, Hao1; Zhao, Yi1; Sun, Zhenjun1,2

2021-01-29

10.1038/s42003-021-01659-4

COMMUNICATIONS BIOLOGY   影响因子和分区

2399-3642

Earthworms (Annelida: Crassiclitellata) are widely distributed around the world due to their ancient origination as well as adaptation and invasion after introduction into new habitats over the past few centuries. Herein, we report a 1.2Gb complete genome assembly of the earthworm Amynthas corticis based on a strategy combining third-generation long-read sequencing and Hi-C mapping. A total of 29,256 protein-coding genes are annotated in this genome. Analysis of resequencing data indicates that this earthworm is a triploid species. Furthermore, gene family evolution analysis shows that comprehensive expansion of gene families in the Amynthas corticis genome has produced more defensive functions compared with other species in Annelida. Quantitative proteomic iTRAQ analysis shows that expression of 147 proteins changed in the body of Amynthas corticis and 16S rDNA sequencing shows that abundance of 28 microorganisms changed in the gut of Amynthas corticis when the earthworm was incubated with pathogenic Escherichia coli O157:H7. Our genome assembly provides abundant and valuable resources for the earthworm research community, serving as a first step toward uncovering the mysteries of this species, and may provide molecular level indicators of its powerful defensive functions, adaptation to complex environments and invasion ability. Xing Wang et al. report a 1.2Gb complete genome assembly of the earthworm Amynthas corticis and demonstrate that it is a triploid species. Gene family evolution, proteomic iTRAQ analysis, and 16S rDNA sequencing demonstrate the evolution of adaptive defensive functions relative to other species in Annelida.

SCI

英语

其他

National Natural Science Foundation of China[31172091,31801190] ; Fundamental Research Funds for the Central Universities["2018QC155","2018ZH003"] ; program of Excellent Youth from Hebei Educational Department and Provincial Natural Fund["BJ2017027","C2019408050"]

Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics

Biology ; Multidisciplinary Sciences

WOS:000616761400002

NATURE RESEARCH

2-s2.0-85100105550

Web of Science

1.China Agr Univ, Coll Resources & Environm Sci, Beijing, Peoples R China
2.Beijing Key Lab Biodivers & Organ Farming, Beijing, Peoples R China
3.Southern Univ Sci & Technol, Sch Environm Sci & Engn, Shenzhen, Guangdong, Peoples R China
4.Langfang Normal Univ, Hebei Key Lab Anim Divers, Langfang, Hebei, Peoples R China
5.China Agr Univ, Coll Biol Sci, Beijing, Peoples R China
6.Maharishi Univ Management, Fairfield, IA USA
7.Beijing Gencode Diagnost Lab, Beijing, Peoples R China
8.Tianjin Chengjian Univ, Sch Environm & Municipal Engn, Tianjin, Peoples R China

Wang, Xing,Zhang, Yi,Zhang, Yufeng,et al. Amynthas corticis genome reveals molecular mechanisms behind global distribution[J]. COMMUNICATIONS BIOLOGY,2021,4(1).

Wang, Xing.,Zhang, Yi.,Zhang, Yufeng.,Kang, Mingming.,Li, Yuanbo.,...&Sun, Zhenjun.(2021).Amynthas corticis genome reveals molecular mechanisms behind global distribution.COMMUNICATIONS BIOLOGY,4(1).

Wang, Xing,et al."Amynthas corticis genome reveals molecular mechanisms behind global distribution".COMMUNICATIONS BIOLOGY 4.1(2021).