A Novel Lipoate-Protein Ligase, Mhp-LplJ, Is Required for Lipoic Acid Metabolism in Mycoplasma hyopneumoniae

Jin, Jin1,2; Chen, Huan3; Wang, Ning1; Zhu, Kemeng1; Liu, Huanhuan4; Shi, Dongfang2; Xin, Jiuqing1; Liu, Henggui1

2021-01-18

10.3389/fmicb.2020.631433

Frontiers in Microbiology   影响因子和分区

1664-302X

Lipoic acid is a conserved cofactor necessary for the activation of several critical enzyme complexes in the aerobic metabolism of 2-oxoacids and one-carbon metabolism. Lipoate metabolism enzymes are key for lipoic acid biosynthesis and salvage. In this study, we found that Mycoplasma hyopneumoniae (M. hyopneumoniae) Mhp-Lpl, which had been previously shown to have lipoate-protein ligase activity against glycine cleavage system H protein (GcvH) in vitro, did not lipoylate the lipoate-dependent subunit of dihydrolipoamide dehydrogenase (PdhD). Further studies indicated that a new putative lipoate-protein ligase in M. hyopneumoniae, MHP_RS00640 (Mhp-LplJ), catalyzes free lipoic acid attachment to PdhD in vitro. In a model organism, Mhp-LplJ exhibited lipoate and octanoate ligase activities against PdhD. When the enzyme activity of Mhp-LplJ was disrupted by lipoic acid analogs, 8-bromooctanoic acid (8-BrO) and 6,8-dichlorooctanoate (6,8-diClO), M. hyopneumoniae growth was arrested in vitro. Taken together, these results indicate that Mhp-LplJ plays a vital role in lipoic acid metabolism of M. hyopneumoniae, which is of great significance to further understand the metabolism of M. hyopneumoniae and develop new antimicrobials against it.

lipoate-protein ligase lipoic acid Mycoplasma hyopneumoniae dihydrolipoamide dehydrogenase lipoic acid analogs

SCI

英语

其他

Central Public-interest Scientific Institution Basal Research Fund[1610302017014]

Microbiology

Microbiology

WOS:000613148700001

FRONTIERS MEDIA SA

Web of Science

1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin, Peoples R China
2.Northeast Agr Univ, Dept Vet Prevent Med, Coll Vet Med, Harbin, Peoples R China
3.Southern Univ Sci & Technol, Guangdong Prov Key Lab Cell Microenvironm & Dis R, Shenzhen Key Lab Cell Microenvironm & SUSTech HKU, Dept Biol, Shenzhen, Peoples R China
4.Yangtze Univ, Coll Life Sci, Kingchow, Peoples R China

Jin, Jin,Chen, Huan,Wang, Ning,et al. A Novel Lipoate-Protein Ligase, Mhp-LplJ, Is Required for Lipoic Acid Metabolism in Mycoplasma hyopneumoniae[J]. Frontiers in Microbiology,2021,11.

Jin, Jin.,Chen, Huan.,Wang, Ning.,Zhu, Kemeng.,Liu, Huanhuan.,...&Liu, Henggui.(2021).A Novel Lipoate-Protein Ligase, Mhp-LplJ, Is Required for Lipoic Acid Metabolism in Mycoplasma hyopneumoniae.Frontiers in Microbiology,11.

Jin, Jin,et al."A Novel Lipoate-Protein Ligase, Mhp-LplJ, Is Required for Lipoic Acid Metabolism in Mycoplasma hyopneumoniae".Frontiers in Microbiology 11(2021).