Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjogren syndrome patients

Huang, Shaoying1; Zheng, Fengping1; Liu, Lixiong2; Meng, Shuhui1; Cai, Wanxia1; Zhang, Cantong1; Dai, Weier4; Liu, Dongzhou2; Hong, Xiaoping2; Tang, Donge1; Dai, Yong1,3

2021-01-15

Aging-US   影响因子和分区

1945-4589

Primary Sjogren syndrome (pSS) is a common autoimmune disease. Here, we performed the first proteome and phosphoproteome analyses of peripheral blood mononuclear cells in pSS patients to obtain a comprehensive profile and identify the potential crucial proteins and pathways for the screening and evaluation of pSS patients. Peripheral blood mononuclear cells from 8 pSS-confirmed patients (American-European Consensus Group Criteria, 2002) and 10 normal controls were selected. Label-free quantitative proteomics was utilized to obtain quantitative information. In total, 787 proteins were identified as differentially expressed proteins, and 175 phosphosites on 123 proteins were identified as differentially phosphorylated proteins. We performed functional enrichment analyses with these proteins and phosphoproteins based on public database. Furthermore, protein-protein interaction network analyses were performed by using multiple algorithms. Using module and hub protein analyses, we identified 16 modules for the proteins, 2 clusters for the phosphoproteins and selected the top 10 hub proteins. Finally, we identified 22 motifs using motif analysis of the phosphosites and found 17 newly identified motifs, while 6 motifs were experimentally verified for known protein kinases. The findings distinguished pSS patients from normal controls at the peripheral blood mononuclear cells level and revealed potential candidates for use in pSS diagnosis.

primary Sjogren syndrome peripheral blood mononuclear cells phosphoproteome biomarker

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[81671596,"8197 1464"] ; Natural Science Foundation of Guangxi[2017GXNSFA A198375] ; National Science Foundation for Young Scientists of China[31700795] ; Key Research and Development Program of Guangdong Province[2019B020229001] ; Shenzhen Key Medical Discipline Construction Fund[SZXK011] ; Guangxi Key Laboratory of Metabolic Diseases Research[20-065-76]

Cell Biology ; Geriatrics & Gerontology

Cell Biology ; Geriatrics & Gerontology

WOS:000611141300005

IMPACT JOURNALS LLC

Web of Science

1.Jinan Univ, Guangdong Prov Engn Res Ctr Autoimmune Dis Precis, Dept Clin Med Res Ctr,Southern Univ Sci & Technol, Shenzhen Peoples Hosp,Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Shenzhen Peoples Hosp, Dept Rheumatol & Immunol, Clin Med Coll 2,Jinan Univ,Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Nephrol Dept Guilin, Guangxi Key Lab Metab Dis Res, Guilin 541002, Peoples R China
4.Univ Texas Austin, Coll Nat Sci, Austin, TX 78712 USA

Huang, Shaoying,Zheng, Fengping,Liu, Lixiong,et al. Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjogren syndrome patients[J]. Aging-US,2021,13(1).

Huang, Shaoying.,Zheng, Fengping.,Liu, Lixiong.,Meng, Shuhui.,Cai, Wanxia.,...&Dai, Yong.(2021).Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjogren syndrome patients.Aging-US,13(1).

Huang, Shaoying,et al."Integrated proteome and phosphoproteome analyses of peripheral blood mononuclear cells in primary Sjogren syndrome patients".Aging-US 13.1(2021).