南方科技大学知识苑(SUSTech KC): Application of electrical treatment on Euglena gracilis for increasing paramylon production

题名	Application of electrical treatment on Euglena gracilis for increasing paramylon production
作者	Kim, Jee Young 1; Oh, Jeong-Joo 1; Kim, Da Hee 1; Kim, Hyun Soo 2; Lee, Changsu 3; Park, Jaewon4 ; Choi, Yoon-E 1
通讯作者	Park, Jaewon; Choi, Yoon-E
发表日期	2021-02-01
DOI	10.1007/s00253-020-11033-9
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 影响因子和分区
ISSN	0175-7598
EISSN	1432-0614
卷号	105 期号:3页码:1031-1039
摘要	Paramylon also called beta-1,3-glucan is a value-added product produced from Euglena gracilis. Recently, researchers have developed various strategies for the enhanced paramylon production, among which electrical treatment for microbial stimulation can be an alternative owing to the applicability to large-scale cultivation. In this study, we applied the electrical treatment for enhanced paramylon production and found the proper treatment conditions. Under the treatment with platinum electrodes at 10 mA, the paramylon production of treated cells was significantly increased about 2.5-fold, compared to those of the untreated cells, although the density of cells was maintained due to considerable stress. The size of treated cells became larger, possibly due to the increased level of paramylon production within the cells. Accordingly, the contents of glucose uptake, glucose-6-phosphate (G6P), glucose-1-phosphate (G1P), and uridine diphosphoglucose (UDPG) were shifted to appropriate states for the process of paramylon synthesis under the treatment. The increased level of transcripts encoding glucan synthase-like 2 (EgGSL2) was also confirmed via droplet digital PCR (ddPCR) under the treatment. Overall, this study makes a major contribution to research on electrical stimulation and provides new insights into E. gracilis metabolism like paramylon synthesis.
关键词	Electrical stimulation Paramylon Euglena gracilis ddPCR and beta glucan synthases
相关链接	[来源记录]
收录类别	SCI ; EI
语种	英语
学校署名	通讯
资助项目	Marine Biotechnology Program of the Korea Institute of Marine Science and Technology (KIMST) - Ministry of Oceans and Fisheries (MOF)[20170488] ; NRF (National Research Foundation of Korea) - Korean Government[NRF-2019R1A2C2087449] ; Korea Basic Science Institute (KBSI)[C070300] ; National Natural Science Foundation of China (NSFC)[51878309] ; Shenzhen Scientific research grant[JCYJ20180504165801830]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000605882200004
出版者	SPRINGER
EI入藏号	20210209760959
EI主题词	Electrodes
EI分类号	Organic Compounds:804.1
ESI学科分类	BIOLOGY & BIOCHEMISTRY
来源库	Web of Science
引用统计	被引频次[WOS]：9
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221366
专题	工学院_深港微电子学院
作者单位	1.Korea Univ, Div Environm Sci & Ecol Engn, Seoul 02841, South Korea 2.Korea Inst Machinery & Mat, Daegu Res Ctr Med Devices & Rehabil, Daegu 42994, South Korea 3.World Inst Kimchi, Microbiol & Funct Res Grp, Gwangju 61755, South Korea 4.Southern Univ Sci & Technol, Sch Microelect, Shenzhen 518055, Peoples R China
通讯作者单位	深港微电子学院
推荐引用方式 GB/T 7714	Kim, Jee Young,Oh, Jeong-Joo,Kim, Da Hee,et al. Application of electrical treatment on Euglena gracilis for increasing paramylon production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2021,105(3):1031-1039.
APA	Kim, Jee Young.,Oh, Jeong-Joo.,Kim, Da Hee.,Kim, Hyun Soo.,Lee, Changsu.,...&Choi, Yoon-E.(2021).Application of electrical treatment on Euglena gracilis for increasing paramylon production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,105(3),1031-1039.
MLA	Kim, Jee Young,et al."Application of electrical treatment on Euglena gracilis for increasing paramylon production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 105.3(2021):1031-1039.