Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses

Gong，Zhenggang1; Yang，Guangxu1; Song，Junlong2; Zheng，Peitao3; Liu，Jing1; Zhu，Wenyuan2; Huang，Liulian1; Chen，Lihui1; Luo，Xiaolin1,2; Shuai，Li1

2021-12-01

10.1186/s40643-021-00363-9

Bioresources and Bioprocessing   影响因子和分区

2197-4365

Abstract: Lignin deposits formed on the surface of pretreated lignocellulosic substrates during acidic pretreatments can non-productively adsorb costly enzymes and thereby influence the enzymatic hydrolysis efficiency of cellulose. In this article, peanut protein (PP), a biocompatible non-catalytic protein, was separated from defatted peanut flour (DPF) as a lignin blocking additive to overcome this adverse effect. With the addition of 2.5 g/L PP in enzymatic hydrolysis medium, the glucose yield of the bamboo substrate pretreated by phenylsulfonic acid (PSA) significantly increased from 38 to 94% at a low cellulase loading of 5 FPU/g glucan while achieving a similar glucose yield required a cellulase loading of 17.5 FPU/g glucan without PP addition. Similar promotion effects were also observed on the n-pentanol-pretreated bamboo and PSA-pretreated eucalyptus substrates. The promoting effect of PP on enzymatic hydrolysis was ascribed to blocking lignin deposits via hydrophobic and/or hydrogen-bonding interactions, which significantly reduced the non-productive adsorption of cellulase onto PSA lignin. Meanwhile, PP extraction also facilitated the utilization of residual DPF as the adhesive for producing plywood as compared to that without protein pre-extraction. This scheme provides a sustainable and viable way to improve the value of woody and agriculture biomass. Peanut protein, a biocompatible non-catalytic protein, can block lignin, improve enzymatic hydrolysis efficiency and thereby facilitate the economics of biorefinery. Graphical abstract: [Figure not available: see fulltext.]

Adsorption Blocking Enzymatic hydrolysis Lignin deposits Peanut protein Pretreatment

SCI ; EI

英语

其他

WOS:000613516400001

2-s2.0-85100221256

Scopus

1.College of Materials Engineering,Fujian Agriculture and Forestry University,Fuzhou,350002,China
2.Jiangsu Provincial Key Laboratory of Pulp and Paper Science and Technology,Nanjing Forestry University,Nanjing,210037,China
3.Department of Materials Science & Engineering,Southern University of Science and Technology,Shenzhen,518055,China

Gong，Zhenggang,Yang，Guangxu,Song，Junlong,et al. Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses[J]. Bioresources and Bioprocessing,2021,8(1).

Gong，Zhenggang.,Yang，Guangxu.,Song，Junlong.,Zheng，Peitao.,Liu，Jing.,...&Shuai，Li.(2021).Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses.Bioresources and Bioprocessing,8(1).

Gong，Zhenggang,et al."Understanding the promoting effect of non-catalytic protein on enzymatic hydrolysis efficiency of lignocelluloses".Bioresources and Bioprocessing 8.1(2021).