Impact of pmrA on Cronobacter sakazakii planktonic and biofilm cells: A comprehensive transcriptomic study

Xu，Zhenbo1,2,3,4,5; Liu，Ziqi1; Soteyome，Thanapop4; Hua，Jingjing6; Zhang，Liang6; Yuan，Lei7; Ye，Yanrui8; Cai，Zhao9; Yang，Liang9; Chen，Ling1; Harro，Janette M.10; Kjellerup，Birthe Veno11; Liu，Junyan11; Li，Yanyan12

2021-09-01

10.1016/j.fm.2021.103785

FOOD MICROBIOLOGY   影响因子和分区

0740-0020

1095-9998

Cronobacter sakazakii is an emerging opportunistic foodborne pathogen causing rare but severe infections in neonates. Furthermore, the formation of biofilm allows C. sakazakii to persist in different environments. We have demonstrated that the mutator phenotype ascribed to deficiency of the pmrA gene results in more biomass in the first 24 h but less during the post maturation stage (7–14 d) compared with BAA 894. The present study aimed to investigate the regulatory mechanism modulating biofilm formation due to pmrA mutation. The transcriptomic analyses of BAA 894 and s-3 were performed by RNA-sequencing on planktonic and biofilm cells collected at different time points. According to the results, when comparing biofilm to planktonic cells, expression of genes encoding outer membrane proteins, lysozyme, etc. were up-regulated, with LysR family transcriptional regulators, periplasmic proteins, etc. down-regulated. During biofilm formation, cellulose synthase operon genes, flagella-related genes, etc. played essential roles in different stages. Remarkably, pmrA varies the expression of a number of genes related to motility, biofilm formation, and antimicrobial resistance, including srfB, virK, mviM encoding virulence factor, flgF, fliN, etc. encoding flagellar assembly, and marA, ramA, etc. encoding AraC family transcriptional regulators in C. sakazakii. This study provides valuable insights into transcriptional regulation of C. sakazakii pmrA mutant during biofilm formation.

biofilm Cronobacter sakazakii pmrA transcriptome

SCI

英语

其他

WOS:000641583700010

AGRICULTURAL SCIENCES

2-s2.0-85102461609

Scopus

1.School of Food Science and Engineering,Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety,South China University of Technology,Guangzhou,510640,China
2.Department of Clinical Pharmacy and Translational Science,College of Pharmacy,University of Tennessee Health Science Center,Memphis,38103,United States
3.Home Economics Technology,Rajamangala University of Technology Phra Nakhon,Bangkok,Thailand
4.National Institute of Fundamental Studies,Kandy,Hantana Road,Sri Lanka
5.Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center),Guangzhou,510640,China
6.National Engineering Laboratory for Cereal Fermentation Technology,Jiangnan University,Wuxi,1800 Lihu Road,214122,China
7.College of Food Science and Engineering,Yangzhou University,Yangzhou,China
8.School of Biological Science and Engineering,South China University of Technology,Guangzhou,510640,China
9.School of Medicine,Southern University of Science and Technology,Shenzhen,518055,China
10.Department of Microbial Pathogenesis,School of Dentistry,University of Maryland,Baltimore,21201,United States
11.Department of Civil and Environmental Engineering,University of Maryland,College Park,20742,United States
12.Key Laboratory of Structural Biology of Zhejiang Province,School of Life Sciences,Westlake University,Hangzhou,18 Shilongshan Road, Xihu District,310024,China

Xu，Zhenbo,Liu，Ziqi,Soteyome，Thanapop,et al. Impact of pmrA on Cronobacter sakazakii planktonic and biofilm cells: A comprehensive transcriptomic study[J]. FOOD MICROBIOLOGY,2021,98.

Xu，Zhenbo.,Liu，Ziqi.,Soteyome，Thanapop.,Hua，Jingjing.,Zhang，Liang.,...&Li，Yanyan.(2021).Impact of pmrA on Cronobacter sakazakii planktonic and biofilm cells: A comprehensive transcriptomic study.FOOD MICROBIOLOGY,98.

Xu，Zhenbo,et al."Impact of pmrA on Cronobacter sakazakii planktonic and biofilm cells: A comprehensive transcriptomic study".FOOD MICROBIOLOGY 98(2021).