Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry

Chen，Peihua1,2; Shen，Xuefeng1,2; Zhao，Shuainan1,2,3; Liu，Zili1,2,3; Zhu，Qianwen2; Zhu，Tao4; Zhang，Shuli2,3; Li，Yi4; Mao，Lanqun5; Sun，Jianyuan1,2,3,6

2021-06-01

10.1016/j.bios.2021.113143

BIOSENSORS & BIOELECTRONICS   影响因子和分区

0956-5663

1873-4235

Neuronal information is majorly encoded chemically at synapses and the elementary unit of synaptic transmission is the contents of neurotransmitter released from single vesicle. However, the contents of quantal neurotransmitter have never been precisely estimated at synapses, which largely prevent our understanding the nature of quantal neurotransmitter release and its impact on neuronal information processing. In order to break through the technical bottleneck of precisely counting quantal neurotransmitter molecules, we developed a new approach in combination of electrophysiology and electrochemistry to measure intact quantal content of single vesicles. An etched submicro-carbon fiber electrode for electrochemical detection was designed to be enclosed in an electrophysiologically used glass pipette. The glass pipette allowed the electrochemical electrode to access the release site, and amperometric recordings were made within the enclosed space at the electrophysiological loose-patch mode. Our study showed that the intact quantal release could be successfully detected at the dopaminergic varicosities by this loose-patch amperometric measurement in real time with negligible leakage.

Amperometry Loose patch Nerve terminals Neurotransmitters Quantal release

EI ; SCI

英语

其他

National Natural Science Foundation of China[31800902,31871033,31527802] ; Youth Innovation Promotion Association, CAS[2017130] ; National Basic Research Program of China[2013CB835100] ; CAS[YJKYYQ20180028] ; Key Research Program of Frontier Sciences, CAS[QYZ-DYSSW-SMC015] ; NSFC-Guangdong Joint Fund[U20A6005]

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics

Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology

WOS:000761671800002

ELSEVIER ADVANCED TECHNOLOGY

20211110064336

Carbon fibers ; Chemical detection ; Electrochemical electrodes ; Electrophysiology ; Glass

Biomedical Engineering:461.1 ; Medicine and Pharmacology:461.6 ; Chemistry:801 ; Chemical Products Generally:804 ; Glass:812.3

CHEMISTRY

2-s2.0-85102396943

Scopus

1.The Brain Cognition and Brain Disease Institute,Shenzhen Institute of Advanced Technology,Chinese Academy of Sciences (CAS),Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions,Shenzhen,518055,China
2.State Key Laboratory of Brain and Cognitive Sciences,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,CAS,Beijing,100101,China
3.University of CAS,Beijing,100049,China
4.School of Microelectronics,MOE Engineering Research Center of Integrated Circuits for Next Generation Communications,Southern University of Science and Technology,Shenzhen,518055,China
5.Institute of Chemistry,CAS,Beijing,100190,China
6.Center for Parkinson's Disease,Beijing Institute for Brain Disorders,Beijing,100053,China

Chen，Peihua,Shen，Xuefeng,Zhao，Shuainan,et al. Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry[J]. BIOSENSORS & BIOELECTRONICS,2021,181.

Chen，Peihua.,Shen，Xuefeng.,Zhao，Shuainan.,Liu，Zili.,Zhu，Qianwen.,...&Sun，Jianyuan.(2021).Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry.BIOSENSORS & BIOELECTRONICS,181.

Chen，Peihua,et al."Measurement of intact quantal packet of transmitters released from single nerve terminal by loose-patch amperometry".BIOSENSORS & BIOELECTRONICS 181(2021).