南方科技大学知识苑(SUSTech KC): Crystal structure of the BRPF2 PWWP domain in complex with DNA reveals a different binding mode than the HDGF family of PWWP domains

题名	Crystal structure of the BRPF2 PWWP domain in complex with DNA reveals a different binding mode than the HDGF family of PWWP domains
作者	Zhang，Mengmeng 1; Lei，Ming 2; Qin，Su3 ; Dong，Aiping 4; Yang，Ally 5; Li，Yanjun 4; Loppnau，Peter 4; Hughes，Timothy R.5; Min，Jinrong 2,4,6; Liu，Yanli 1
通讯作者	Min，Jinrong
发表日期	2021-03-01
DOI	10.1016/j.bbagrm.2021.194688
发表期刊	Biochimica et Biophysica Acta-Gene Regulatory Mechanisms 影响因子和分区
ISSN	1874-9399
EISSN	1876-4320
卷号	1864 期号:3
摘要	The PWWP domain was first identified in the HDGF protein family and named after the conserved Proline-Tryptophan-Tryptophan-Proline motif in WHSC1. The PWWP domain-containing proteins play important roles in different biological processes, such as DNA replication, transcription, DNA repair, pre-mRNA processing by recognizing methylated histone and dsDNA simultaneously. Recently, how the HDGF family of PWWP domains recognize histone H3K36me3-modified nucleosome has been reported. In order to better understand the interactions between the PWWP domain and dsDNA, we carried out family-wide characterization of dsDNA binding abilities of human PWWP domains. Our binding assays confirmed that PWWP domains bind to dsDNA without sequence selectivity. Our crystal structure of the BRPF2 PWWP domain in complex with a 12-mer dsDNA reveals that the PWWP domain interacts with dsDNA by binding to its major groove, instead of the minor groove observed in the HDGF family of PWWP domains. Our study indicates that PWWP domains could bind to dsDNA in different modes.
关键词	DNA Major groove PWWP domain
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	其他
WOS记录号	WOS:000641019800004
Scopus记录号	2-s2.0-85100727972
来源库	Scopus
引用统计	被引频次[WOS]：13
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221603
专题	南方科技大学生命科学学院
作者单位	1.College of Pharmaceutical Sciences,Soochow University,Suzhou,China 2.Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central China Normal University,Wuhan,China 3.Life Science Research Center,Southern University of Science and Technology,Shenzhen,China 4.Structural Genomics Consortium,University of Toronto,Toronto,Canada 5.Donnelly Centre for Cellular and Biomolecular Research,University of Toronto,Toronto,Canada 6.Department of Physiology,University of Toronto,Toronto,Canada
推荐引用方式 GB/T 7714	Zhang，Mengmeng,Lei，Ming,Qin，Su,et al. Crystal structure of the BRPF2 PWWP domain in complex with DNA reveals a different binding mode than the HDGF family of PWWP domains[J]. Biochimica et Biophysica Acta-Gene Regulatory Mechanisms,2021,1864(3).
APA	Zhang，Mengmeng.,Lei，Ming.,Qin，Su.,Dong，Aiping.,Yang，Ally.,...&Liu，Yanli.(2021).Crystal structure of the BRPF2 PWWP domain in complex with DNA reveals a different binding mode than the HDGF family of PWWP domains.Biochimica et Biophysica Acta-Gene Regulatory Mechanisms,1864(3).
MLA	Zhang，Mengmeng,et al."Crystal structure of the BRPF2 PWWP domain in complex with DNA reveals a different binding mode than the HDGF family of PWWP domains".Biochimica et Biophysica Acta-Gene Regulatory Mechanisms 1864.3(2021).