Detection and analysis of early degradation at resin-dentin interface by optical coherence tomography (OCT) and confocal laser scanning microscope (CLSM)

Zhou，Yuan1; Matin，Khairul2,3; Shimada，Yasushi4; Wang，Guoqing5; Sadr，Alireza6; Tagami，Junji2

2021-03-01

10.1016/j.jdent.2021.103583

JOURNAL OF DENTISTRY   影响因子和分区

0300-5712

Objective: To evaluate early degradation at resin-dentin interface using non-invasive swept-source optical coherence tomography (SS-OCT) and confocal laser scanning microscope (CLSM). Methods: Self-etch adhesives and resin-composites containing bisphenol-glycidyl-dimethacrylate (Bis-GMA), which is one of the most widely used monomers in restorative materials, were investigated in this study. Forty cervical cavities were prepared in bovine incisors and applied by the adhesive with/without Bis-GMA (AdhesiveBG/Adhesive), filled by the resin with/without Bis-GMA (ResinBG/Resin) and then challenged by cariogenic biofilm (37 °C, 24 h). Gap Formation and dentin demineralization around resin-composites were observed by SS-OCT and CLSM. Results: Three types of resin-dentin interfacial degradation could be detected from SS-OCT. Type I—dentin demineralization around resin without gap, showing feather-shaped dark zones without bright scattered lines at resin-dentin interfaces. Type II—dentin demineralization around resin with adhesive-dentin bonded gaps, showing feather-shaped dark zones with bright scattered lines at resin-dentin interfaces. Type III—dentin demineralization around resin with adhesive-dentin debonded gaps, showing edge-shaped dark zones with bright scattered lines at resin-dentin interface. From CLSM, the groups were compared in gap scale (GS), gap depth (GD), gap width (GW) and dentin wall lesion depth (WLD). Bis-GMA-containing adhesive groups showed significantly lower GS than Bis-GMA-free adhesive groups. Bis-GMA-containing resin groups showed significantly lower WLD than Bis-GMA-free resin groups. However, they did not show significant differences in GD and GW. Conclusion: Three types of early degradation at resin-dentin interface can be noninvasively detected by SS-OCT. Bis-GMA-containing and Bis-GMA-free restorative materials show differences in gap scale and dentin wall lesion depth. Clinical significance: SS-OCT can nondestructively detect early resin-dentin interfacial degradation. Gap scale can be used as a parameter to evaluate the risk factor of gaps.

Confocal laser scanning microscope Degradation Gap Optical coherence tomography Restorative material Secondary caries

SCI

英语

其他

WOS:000636366300006

CLINICAL MEDICINE

2-s2.0-85099630239

Scopus

1.Laboratory of Molecular and Preventive Dentistry,Department of Preventive Dentistry,Shanghai Ninth People's Hospital,Shanghai JiaoTong University School of Medicine,Shanghai,639 Zhizaoju Road, Huangpu District,200011,China
2.Department of Cariology and Operative Dentistry,Graduate School of Medical and Dental Sciences,Tokyo Medical and Dental University,Tokyo,Japan
3.Endowed Department of International Oral Health Science (Affiliated With Department of Translational Research),School of Dental Medicine,Tsurumi University,Yokohama,Kanagawa,Japan
4.Department of Operative Dentistry,Graduate School of Medicine,Dentistry and Pharmaceutical Sciences,Okayama University,Okayama,Japan
5.Department of Electrical and Electronic Engineering,Southern University of Science and Technology,Shenzhen,China
6.Biomimetics Biomaterials Biophotonics & Technology Laboratory,Department of Restorative Dentistry,University of Washington School of Dentistry,Seattle,United States

Zhou，Yuan,Matin，Khairul,Shimada，Yasushi,et al. Detection and analysis of early degradation at resin-dentin interface by optical coherence tomography (OCT) and confocal laser scanning microscope (CLSM)[J]. JOURNAL OF DENTISTRY,2021,106.

Zhou，Yuan,Matin，Khairul,Shimada，Yasushi,Wang，Guoqing,Sadr，Alireza,&Tagami，Junji.(2021).Detection and analysis of early degradation at resin-dentin interface by optical coherence tomography (OCT) and confocal laser scanning microscope (CLSM).JOURNAL OF DENTISTRY,106.

Zhou，Yuan,et al."Detection and analysis of early degradation at resin-dentin interface by optical coherence tomography (OCT) and confocal laser scanning microscope (CLSM)".JOURNAL OF DENTISTRY 106(2021).