Polymorphisms and Circulating Plasma Protein Levels of Immune Checkpoints (CTLA-4 and PD-1) Are Associated With Posner-Schlossman Syndrome in Southern Chinese

Huang，Xiaosheng1,2; Liu，Xinhua1,2; Ye，Ye2; Zhang，Tong1; Mei，Shaoyi1,2; Zhu，Tianhui1,2; Peng，Shiming1,2; Cai，Jiamin2; Yan，Zonghui1,2; Zeng，Kun1,2; Nie，Danyao1,2; Sun，Liangnan1,2; Hou，Xiaofeng1; Zhao，Jun3

2021-02-24

10.3389/fimmu.2021.607966

Frontiers in Immunology   影响因子和分区

1664-3224

1664-3224

Cytotoxic T-lymphocyte associated protein 4 (CTLA-4) and programmed cell death 1 (PD-1) are well-known key immune checkpoints that play a crucial dampening effect on regulating T-cell homeostasis and self-tolerance. In this study, we aimed to evaluate the association between immune checkpoints (CTLA-4 and PD-1) and Posner-Schlossman syndrome (PSS) in a southern Chinese population. A total of 137 patients with PSS and 139 healthy controls from a southern Chinese population were recruited. Five single nucleotide polymorphisms (SNPs) of CTLA-4 (rs733618, rs4553808, rs5742909, rs231775, and rs3087243) and five SNPs of PD-1 (rs10204525, rs2227981, rs2227982, rs41386349, and rs36084323) were genotyped by SNaPshot technique. Soluble CTLA-4 (sCTLA-4) and soluble PD-1 (sPD-1) were determined by ELISA and antibody array assay, respectively. The frequencies of T allele at rs733618 and A allele at rs231775 of CTLA-4 were significantly higher in PSS patients than in healthy controls (corrected p (P) = 0.037; P = 0.044, respectively). The haplotype frequencies of CACGG haplotype (rs733618-rs4553808-rs5742909-rs231775-rs3087243) of CTLA-4 and TGAGC haplotype (rs10204525-rs2227981-rs2227982-rs41386349-rs36084323) of PD-1 in the PSS group was significantly lower than those in the control group (P = 0.015, p = 0.034, respectively). Circulating plasma levels of sCTLA-4 and sPD-1 in PSS patients were significantly higher than those in controls (all p < 0.001). The present study suggests that CTLA-4 and PD-1 genetic polymorphisms are associated with the susceptibility to PSS in a southern Chinese population. The upregulated circulating plasma protein levels of sCTLA-4 and sPD-1 might provide some hints regarding the dysfunction of immune checkpoints in PSS during the active status.

CTLA-4 genetic variants PD-1 Posner-Schlossman syndrome soluble molecular

SCI

英语

通讯

Science, Technology, and Innovation Commission of Shenzhen Municipality["JCYJ20180228164400218","GJHZ20180420180937076"] ; Sanming Project of Medicine in Shenzhen Grant[SZSM201812090]

Immunology

Immunology

WOS:000626735500001

FRONTIERS MEDIA SA

2-s2.0-85102431210

Scopus

1.Shenzhen Eye Institute,Shenzhen Eye Hospital,Jinan University,Shenzhen,China
2.School of Ophthalmology,Optometry,Shenzhen Eye Hospital,Shenzhen University,Shenzhen,China
3.Department of Ophthalmology,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,China

Huang，Xiaosheng,Liu，Xinhua,Ye，Ye,et al. Polymorphisms and Circulating Plasma Protein Levels of Immune Checkpoints (CTLA-4 and PD-1) Are Associated With Posner-Schlossman Syndrome in Southern Chinese[J]. Frontiers in Immunology,2021,12.

Huang，Xiaosheng.,Liu，Xinhua.,Ye，Ye.,Zhang，Tong.,Mei，Shaoyi.,...&Zhao，Jun.(2021).Polymorphisms and Circulating Plasma Protein Levels of Immune Checkpoints (CTLA-4 and PD-1) Are Associated With Posner-Schlossman Syndrome in Southern Chinese.Frontiers in Immunology,12.

Huang，Xiaosheng,et al."Polymorphisms and Circulating Plasma Protein Levels of Immune Checkpoints (CTLA-4 and PD-1) Are Associated With Posner-Schlossman Syndrome in Southern Chinese".Frontiers in Immunology 12(2021).