Cortical recruitment of centralspindlin and rhoa effectors during meiosis i of caenorhabditis elegans primary spermatocytes

Wang，Xiangchuan1,2; Zhang，Dandan1,2; Zheng，Cunni1,2; Wu，Shian3; Glotzer，Michael4; Tse，Yu Chung1,2,5

2021-02-01

10.1242/jcs.238543

JOURNAL OF CELL SCIENCE   影响因子和分区

0021-9533

1477-9137

Haploid male gametes are produced through meiosis during gametogenesis. Whereas the cell biology of mitosis and meiosis is well studied in the nematode Caenorhabditis elegans, comparatively little is known regarding the physical division of primary spermatocytes during meiosis I. Here, we investigated this process using high-resolution timelapse confocal microscopy and examined the spatiotemporal regulation of contractile ring assembly in C. elegans primary spermatocytes. We found that centralspindlin and RhoA effectors were recruited to the equatorial cortex of dividing primary spermatocytes for contractile ring assembly before segregation of homologous chromosomes. We also observed that perturbations shown to promote centralspindlin oligomerization regulated the cortical recruitment of NMY-2 and impacted the order in which primary spermatocytes along the proximal-distal axis of the gonad enter meiosis I. These results expand our understanding of the cellular division of primary spermatocytes into secondary spermatocytes during meiosis I.

Centralspindlin Contractile ring Meiosis RhoA Spermatocyte

SCI

英语

第一 ; 通讯

National Natural Science Foundation of China[31671513,"R35GM127091"] ; Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research[2017B030301018] ; Natural Science Foundation of Guangdong Province[2020A1515010742] ; Shenzhen Science and Technology Innovation Program[JCYJ20180305123513346]

Cell Biology

Cell Biology

WOS:000623042600006

COMPANY BIOLOGISTS LTD

MOLECULAR BIOLOGY & GENETICS

2-s2.0-85101536033

Scopus

1.Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
2.Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research,Southern University of Science and Technology,Shenzhen,518055,China
3.State Key Laboratory of Medicinal Chemical Biology,College of Life Sciences,Nankai University,Tianjin,300071,China
4.Department of Molecular Genetics and Cell Biology,University of Chicago,Chicago,60637,United States
5.Core Research Facilities,Southern University of Science and Technology,Shenzhen,518055,China

Wang，Xiangchuan,Zhang，Dandan,Zheng，Cunni,et al. Cortical recruitment of centralspindlin and rhoa effectors during meiosis i of caenorhabditis elegans primary spermatocytes[J]. JOURNAL OF CELL SCIENCE,2021,134(3).

Wang，Xiangchuan,Zhang，Dandan,Zheng，Cunni,Wu，Shian,Glotzer，Michael,&Tse，Yu Chung.(2021).Cortical recruitment of centralspindlin and rhoa effectors during meiosis i of caenorhabditis elegans primary spermatocytes.JOURNAL OF CELL SCIENCE,134(3).

Wang，Xiangchuan,et al."Cortical recruitment of centralspindlin and rhoa effectors during meiosis i of caenorhabditis elegans primary spermatocytes".JOURNAL OF CELL SCIENCE 134.3(2021).