Near-Infrared-II Nanoparticles for Cancer Imaging of Immune Checkpoint Programmed Death-Ligand 1 and Photodynamic/Immune Therapy

Liu，Qiang1,3; Tian，Jiangwei4; Tian，Ye5; Sun，Qinchao6; Sun，Dan7; Wang，Feifei3; Xu，Haijun7; Ying，Guoliang2,8; Wang，Jigang1,9; Yetisen，Ali K.10; Jiang，Nan2,11

2021-01-26

10.1021/acsnano.0c05317

ACS Nano   影响因子和分区

1936-0851

1936-086X

Development of second near-infrared (NIR-II) nanoparticles (NPs) with high biocompatibility, low toxicity, and high singlet oxygen quantum yield (φΔ) to prevent tumor recurrence is highly desirable in molecular imaging and photodynamic/immune combination therapy. Here, theranostic photosensitizer BODIPY (BDP)-I-N-anti-PD-L1 NPs were developed by encapsulating the photosensitizer BDP-I-N with amphipathic poly(styrene-co-chloromethylstyrene)-graft-poly(ethylene glycol) nanocarriers through self-assembly functionalization with programmed cell death-ligand 1 (PD-L1) monoclonal antibody. These NPs exhibit highly intensive luminescence in the NIR-II window (1000-1700 nm) to real-time imaging of immune checkpoint PD-L1, high singlet oxygen quantum yield (φΔ = 73%), and an eliminating effect of primary cancers. The NPs also allow for profiling PD-L1 expression as well as accumulating in MC38 tumor and enabling molecular imaging in vivo. Upon an 808 nm laser excitation, the targeted NPs produce an emission wavelength above 1200 nm to image a tumor to a normal tissue signal ratio (T/NT) at an approximate value of 14.1. Moreover, the MC38 tumors in mice are eliminated by combining photodynamic therapy and immunotherapy within 30 days, with no tumor recurrence within a period of 40 days. In addition, the tumors do not grow in the rechallenged mice within 7 days of inoculation. Such a strategy shows a durable immune memory effect against tumor rechallenging without toxic side effects to major organs.

immunotherapy molecular imaging NIR-II fluorophore probe photodynamic therapy singlet oxygen quantum yield

SCI ; EI

英语

NI论文

通讯

National Natural Science Foundation of China[22077135] ; Natural Science Foundation of Jiangsu Province[BK20151513]

Chemistry ; Science & Technology - Other Topics ; Materials Science

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary

WOS:000613942700033

AMER CHEMICAL SOC

20210509853295

Biocompatibility ; Cell death ; Diseases ; Infrared devices ; Laser excitation ; Ligands ; Mammals ; Molecular imaging ; Molecular oxygen ; Monoclonal antibodies ; Nanoparticles ; Photosensitizers ; Polyethylene glycols ; Quantum yield ; Self assembly ; Styrene ; Theranostics ; Tumors

Bioengineering and Biology:461 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Nanotechnology:761 ; Physical Chemistry:801.4 ; Organic Compounds:804.1 ; Inorganic Compounds:804.2 ; Organic Polymers:815.1.1 ; Solid State Physics:933 ; Materials Science:951

2-s2.0-85100053025

Scopus

1.Artemisinin Research Center and Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing,100700,China
2.West China School of Basic Medical Sciences and Forensic Medicine,Sichuan University,Chengdu,610041,China
3.Department of Chemistry,Stanford University,Stanford,94305,United States
4.State Key Laboratory of Natural Medicines,Jiangsu Key Laboratory of Tcm Evaluation and Translational Research,School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing,211198,China
5.Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering,Biomaterials Research Center,School of Biomedical Engineering,Southern Medical University,Guangzhou,510515,China
6.Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology,Center for Biomedical Optics and Molecular Imaging,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,China
7.Jiangsu Co-innovation Center of Efficient Processing and Utilization of Forest Resources,College of Chemical Engineering,Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals,Nanjing Forestry University,Nanjing,210037,China
8.Brigham and Women's Hospital,Harvard Medical School,Cambridge,02139,United States
9.Department of Urology,Shenzhen People's Hospital (The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,518020,China
10.Department of Chemical Engineering,Imperial College London,London,SW7 2AZ,United Kingdom
11.School of Engineering and Applied Sciences,Harvard University,Cambridge,02138,United States

Liu，Qiang,Tian，Jiangwei,Tian，Ye,et al. Near-Infrared-II Nanoparticles for Cancer Imaging of Immune Checkpoint Programmed Death-Ligand 1 and Photodynamic/Immune Therapy[J]. ACS Nano,2021,15(1):515-525.

Liu，Qiang.,Tian，Jiangwei.,Tian，Ye.,Sun，Qinchao.,Sun，Dan.,...&Jiang，Nan.(2021).Near-Infrared-II Nanoparticles for Cancer Imaging of Immune Checkpoint Programmed Death-Ligand 1 and Photodynamic/Immune Therapy.ACS Nano,15(1),515-525.

Liu，Qiang,et al."Near-Infrared-II Nanoparticles for Cancer Imaging of Immune Checkpoint Programmed Death-Ligand 1 and Photodynamic/Immune Therapy".ACS Nano 15.1(2021):515-525.