Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen

Liu，Pengfei1,2,3; Chen，Shubin1,4; Wang，Yaofeng1,4,5,6; Chen，Xiaoming1,4; Guo，Yiping1; Liu，Chunhua1,4; Wang，Haitao1; Zhao，Yifan1,4,7; Wu，Di1,7; Shan，Yongli1; Zhang，Jian1; Wu，Chuman1; Li，Dongwei1; Zhang，Yanmei1,4; Zhou，Tiancheng1; Chen，Yaoyu1,7; Liu，Xiaobo1,7; Li，Chenxu1,7; Wang，Lihui8; Jia，Bei9; Liu，Jie10; Feng，Bo5; Cai，Jinglei1,6,11; Pei，Duanqing1,4,6,11

2021

10.1007/s11427-020-1897-0

Science China-Life Sciences   影响因子和分区

1674-7305

1869-1889

A stable, rapid and effective neural differentiation method is essential for the clinical applications of human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in treating neurological disorders and diseases. Herein, we established a novel and robust monolayer differentiation method to produce functional neural progenitor cells (NPCs) from human ESC/iPSCs on Type I Collagen. The derived cells not only displayed the requisite markers, but also behaved similarly to classic NPCs both in vitro and in vivo. Upon transplantation into traumatic brain injury model, the derived NPCs facilitated recovery from injury. We also found that SMAD signaling stayed down throughout the differentiation process on Type I Collagen, and the pluripotent signals were rapidly downregulated along with raising up of neural early markers on the third day. Meanwhile, ATAC-seq data showed the related mediation of distinct transcriptome and global chromatin dynamics during NPC induction. Totally, our results thus provide a convenient way to generate NPCs from human ESC/iPSCs for neural diseases’ treatment.

ESCs iPSCs neural differentiation NPCs Type I Collagen

SCI

英语

其他

WOS:000630689400001

2-s2.0-85102741168

Scopus

1.CAS Key Laboratory of Regenerative Biology,Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine,Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences,Guangzhou,510530,China
2.Ambulatory Surgical Center,The 2nd Clinical medical College (Shenzhen People’s Hospital) of Jinan University,The 1st Affiliated Hospitals of Southern University of Science and Technology,Shenzhen,518020,China
3.Integrated Chinese and Western Medicine Postdoctoral Research Station,Jinan University,Guangzhou,510632,China
4.Bioland Laboratory,Guangzhou Regenerative Medicine and Health Guangdong Laboratory,Guangzhou,510005,China
5.School of Biomedical Sciences,The Chinese University of Hong Kong,Hong Kong
6.Centre for Regenerative Medicine and Health,Hong Kong Institute of Science & Innovation,Chinese Academy of Sciences,Hong Kong
7.Department of Regenerative Medicine,School of Pharmaceutical Science,Jilin University,Changchun,130012,China
8.Department of Pathology,Medical College,Jinan University,Guangzhou,510632,China
9.The Center for Prenatal and Hereditary Disease Diagnosis,Department of Obstetrics and Gynecology,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China
10.Department of Obstetrics and Gynecology,Nanfang Hospital,Southern Medical University,Guangzhou,510515,China
11.Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing,100101,China

Liu，Pengfei,Chen，Shubin,Wang，Yaofeng,et al. Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen[J]. Science China-Life Sciences,2021.

Liu，Pengfei.,Chen，Shubin.,Wang，Yaofeng.,Chen，Xiaoming.,Guo，Yiping.,...&Pei，Duanqing.(2021).Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen.Science China-Life Sciences.

Liu，Pengfei,et al."Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen".Science China-Life Sciences (2021).