南方科技大学知识苑(SUSTech KC): Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis

题名	Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis
作者	Li，Yin Peng 1; Du，Xian Rong 1; Zhang，Ru 2; Yang，Qiu 1
发表日期	2021
DOI	10.1080/21655979.2021.1880717
发表期刊	Bioengineered 影响因子和分区
ISSN	2165-5979
EISSN	2165-5987
卷号	12 期号:1页码:763-778
摘要	Interleukin (IL)-18 has a clear antitumor effect; however, its mechanisms of action are not understood in patients with colorectal cancer (CRC). Here, we investigated the potential mechanism of IL-18 in CRC. The results showed that IL-18 treatment alone had no effect on HCT116 cells apoptosis, whereas IL-18 in the presence of natural killer (NK) cells resulted in apoptosis and inhibition of cells proliferation in vitro. Profiling of miRNA expression following coculture with NK cells and treatment with IL-18 resulted in significant downregulation of miR-574-3p expression and upregulated expression of the target gene transforming growth factor beta 1 (TGF-β1). miR-574-3p binds to TGF-β1, and miR-574-3p overexpression increased the proliferation and decreased the apoptotic rate of HCT116 cells in NK cells coculture with IL-18 treatment; overexpression of TGF-β1 restored the effect of miR-574-3p overexpression. The miRNA profile of HCT116 undergoes significant alteration before and after coculturing with NK cells and treatment with IL-18. IL-18 alone did not affect HCT116 cells apoptosis but did promote the antitumor ability of NK cells in coculture with HCT116 cells via the miR-574-3p/TGF-β1 axis. Our study suggested that IL-18 can be a new potential target for cancer immunotherapy for CRC.
关键词	colorectal cancer IL-18 microRNA miR-574-3p natural killer cells tgf-β1
相关链接	[Scopus记录]
收录类别	SCI
语种	英语
学校署名	第一
资助项目	Shenzhen Basic Research Project[JCYJ20160422143629104]
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000627013400001
出版者	TAYLOR & FRANCIS INC
Scopus记录号	2-s2.0-85102191018
来源库	Scopus
引用统计	被引频次[WOS]：14
成果类型	期刊论文
条目标识符	http://sustech.caswiz.com/handle/2SGJ60CL/221759
专题	南方科技大学第一附属医院
作者单位	1.Department of Gastroenterology,Shenzhen People’s Hospital Longhua Branch,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,518020,China 2.Department of Gastroenterology,Department of Gastroenterology,Shenzhen People’s Hospital,The Second Clinical Medical College,Jinan University,The First Affiliated Hospital,Southern University of Science and Technology),Shenzhen,518020,China
第一作者单位	南方科技大学第一附属医院
第一作者的第一单位	南方科技大学第一附属医院
推荐引用方式 GB/T 7714	Li，Yin Peng,Du，Xian Rong,Zhang，Ru,et al. Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis[J]. Bioengineered,2021,12(1):763-778.
APA	Li，Yin Peng,Du，Xian Rong,Zhang，Ru,&Yang，Qiu.(2021).Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis.Bioengineered,12(1),763-778.
MLA	Li，Yin Peng,et al."Interleukin-18 promotes the antitumor ability of natural killer cells in colorectal cancer via the miR-574-3p/TGF-β1 axis".Bioengineered 12.1(2021):763-778.