Metabolomics and lipidomics analyses delineating Hfq deletion- induced metabolic alterations in Vibrio alginolyticus

Zhao, Yanni1; Ren, Jiamin1; Jiang, Heyang1; Chen, Xuefeng1; Xu, Mudan1; Li, Yue1; Zhao, Jieyu3; Chen, Dan1; Zhang, Kun1; Li, Hua2; Liu, Huan1

2021-03-30

10.1016/j.aquaculture.2021.736349

AQUACULTURE   影响因子和分区

0044-8486

1873-5622

Vibrio alginolyticus, a Gram-negative halophilic zoonotic pathogen, can cause bacterial hemorrhagic septicemia in various aquaculture species and wound infections in humans. V. alginolyticus brings about huge economic losses in aquaculture all around the world. Hfq, a ubiquitous RNA-binding protein, is a global post-transcriptional regulator in most bacteria and some archaea, by facilitating base-pairing between sRNAs and their mRNA targets, and is essential for adaptation to different environments and growth conditions. Despite its important roles in the stress adaptation, virulence, nutrient utilization, and colony morphology in V. alginolyticus, further research is required to shed light on the pathways modulated by Hfq. In this study, we used metabolomics and lipidomics analytical approaches based on ultrahigh-performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) to understand the impact of Hfq on the metabolic profile of V. alginolyticus. The deletion of the hfq gene resulted in the growth deficiency and cell elongation of V. alginolyticus cultured in the rich medium. Substantial metabolic alterations, mainly involved in amino acids, nucleotides, fatty acids, and lipids, were observed in the hfq deletion mutant strain (Delta hfq) when compared to the wild-type (WT) strain and its complemented strain (hfq(+)). Notably, the hfq deletion induced accumulations of free fatty acids (FFAs) and nucleotides, but decreased levels of most amino acids (e.g., branched amino acids, aromatic amino acids, and aspartate family amino acids). Meanwhile, the majority of lipids (e.g., lysophosphatidylglycerol, lysophosphatidylethanolamine, phosphatidylcholine, phosphatidic acid, cardiolipin, sphingomyelin, some phosphatidylglycerol and phosphatidylethanolamine with mono- or poly-unsaturated fatty acids) were decreased, while ceramide, diacylglycerol, triacylglycerol and some phosphatidylglycerol and phosphatidylethanolamine with saturated fatty acids increased in the Delta hfq strain. These data demonstrated that the hfq deletion triggered de novo synthesis of FFAs and nucleosides and enhanced the catabolism of phospholipid and sphingomyelin to further regulate cell division and growth. Additionally, the hfq deletion could inhibit the synthesis of the branched amino acids, aromatic amino acids and enhance the TCA cycle to influence cell stress defenses and energy metabolism. Our research indicates that Hfq can regulate pathways associated with cell division, growth and stress resistance, being an important metabolic modulator in V. alginolyticus.

Vibrio alginolyticus Hfq Metabolomics Lipidomics Metabolic alterations

SCI

英语

通讯

National Natural Science Foundation of China[31800328,21904057,32070129] ; Key Research Project of Shaanxi Provincial Science and Technology Department["2020NY-121","2020NY-97"] ; Xi'an City Weiyang District Science and Technology Project[201937,201940] ; Shaanxi University of Science and Technology Doctoral Startup Fund["2016GBJ-11","BJ12-24"] ; Hebei Province Natural Science Foundation[B2019415024]

Fisheries ; Marine & Freshwater Biology

Fisheries ; Marine & Freshwater Biology

WOS:000620270500005

ELSEVIER

PLANT & ANIMAL SCIENCE

Web of Science

1.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China
2.Southern Univ Sci & Technol, SUSTech Core Res Facil, Shenzhen 518055, Peoples R China
3.Hebei Univ Environm Engn, Sch Environm Engn, Qinhuangdao 066102, Hebei, Peoples R China

Zhao, Yanni,Ren, Jiamin,Jiang, Heyang,et al. Metabolomics and lipidomics analyses delineating Hfq deletion- induced metabolic alterations in Vibrio alginolyticus[J]. AQUACULTURE,2021,535.

Zhao, Yanni.,Ren, Jiamin.,Jiang, Heyang.,Chen, Xuefeng.,Xu, Mudan.,...&Liu, Huan.(2021).Metabolomics and lipidomics analyses delineating Hfq deletion- induced metabolic alterations in Vibrio alginolyticus.AQUACULTURE,535.

Zhao, Yanni,et al."Metabolomics and lipidomics analyses delineating Hfq deletion- induced metabolic alterations in Vibrio alginolyticus".AQUACULTURE 535(2021).