Metabolomics analysis of serum in pediatric nephrotic syndrome based on targeted and non-targeted platforms

Guo，Jing1; Zhao，Jinhui1; Liu，Rui2; Yu，Jiaying1; Zhang，Mingjia1; Wang，Hanming3; Liu，Liyan1

2021-04-01

10.1007/s11306-021-01788-1

Metabolomics   影响因子和分区

1573-3882

1573-3890

Background and aims: Nephrotic syndrome (NS) is a common pediatric urinary system disease. The aim in this work was to investigate the changes in pediatric NS-related metabolites through serum metabolomics, and explore the new potential metabolites and differential metabolic pathways. Methods: Serum samples from 40 pediatric patients with nephrotic syndrome and 40 healthy controls were collected. The targeted and non-targeted metabolomics analyses were performed to determine the metabolic changes in pediatric NS. Based on multivariate statistical analysis and the regression model, the serum potential metabolites were screened and different metabolic pathways were explored. Results: 39 differential metabolites in pediatric NS were obtained based on the metabolomics analysis. 12 differential metabolites (serine, C18: 2 (EFA), C18: 2 (FFA), Isonuatigenin 3- [rhamnosyl- (1- > 2) -glucoside], C18: 4 (EFA), C18: 4 (FFA), caprylic acid, citric acid, methylmalonic acid, caproic acid, canavalioside and uroporphyrin were identified to establish the diagnostic model for pediatric NS. Five metabolic pathways including TCA cycle, amino acid metabolism, bile acid biosynthesis, linoleate metabolism and glyoxylate and dicarboxylate metabolism were the key differential metabolic pathways. Conclusion: These data elucidated the metabolic alterations associated with pediatric NS and suggested a new diagnosis model for monitoring pediatric NS. The current study provides the useful information to bridge the gaps in our understanding of the metabolic alterations associated with pediatric NS and might facilitate the characterization of pediatric NS patients by performing serum metabolomics. Graphic Abstract: [Figure not available: see fulltext.]

Different metabolites Metabolomics Pathway analysis Pediatric nephrotic syndrome

SCI

英语

其他

Endocrinology & Metabolism

Endocrinology & Metabolism

WOS:000635634900001

SPRINGER

2-s2.0-85103404416

Scopus

1.Department of Nutrition and Food Hygiene,Public Health College,Harbin Medical University,Harbin,157 Baojian Road, Nangang District,150086,China
2.The Department of Clinical Nutrition,Southern University of Science and Technology Hospital,Shenzhen,China
3.Department of Infectious Diseases,Harbin Children’s Hospital,Harbin,57 Youyi Road, Daoli District,China

Guo，Jing,Zhao，Jinhui,Liu，Rui,et al. Metabolomics analysis of serum in pediatric nephrotic syndrome based on targeted and non-targeted platforms[J]. Metabolomics,2021,17(4).

Guo，Jing.,Zhao，Jinhui.,Liu，Rui.,Yu，Jiaying.,Zhang，Mingjia.,...&Liu，Liyan.(2021).Metabolomics analysis of serum in pediatric nephrotic syndrome based on targeted and non-targeted platforms.Metabolomics,17(4).

Guo，Jing,et al."Metabolomics analysis of serum in pediatric nephrotic syndrome based on targeted and non-targeted platforms".Metabolomics 17.4(2021).