Profiling of sub‐lethal in vitro effects of multi‐walled carbon nanotubes reveals changes in chemokines and chemokine receptors

Keshavan，Sandeep1; Andón，Fernando Torres1,2,3; Gallud，Audrey1,4; Chen，Wei5,6; Reinert，Knut7; Tran，Lang8; Fadeel，Bengt1

2021-04-01

10.3390/nano11040883

Nanomaterials   影响因子和分区

2079-4991

Engineered nanomaterials are potentially very useful for a variety of applications, but studies are needed to ascertain whether these materials pose a risk to human health. Here, we studied three benchmark nanomaterials (Ag nanoparticles, TiO2 nanoparticles, and multi‐walled carbon nanotubes, MWCNTs) procured from the nanomaterial repository at the Joint Research Centre of the European Commission. Having established a sub‐lethal concentration of these materials using two human cell lines representative of the immune system and the lungs, respectively, we performed RNA sequencing of the macrophage‐like cell line after exposure for 6, 12, and 24 h. Downstream analysis of the transcriptomics data revealed significant effects on chemokine signaling pathways. CCR2 was identified as the most significantly upregulated gene in MWCNT‐exposed cells. Using multiplex assays to evaluate cytokine and chemokine secretion, we could show significant effects of MWCNTs on several chemokines, including CCL2, a ligand of CCR2. The results demonstrate the importance of evaluating sub‐lethal concentrations of nanomaterials in relevant target cells.

Chemokines Macrophages Multi‐walled carbon nanotubes Nanoparticles Transcriptomics

SCI

英语

其他

WOS:000643403900001

2-s2.0-85103332147

Scopus

1.Institute of Environmental Medicine,Karolinska Institute,Stockholm,171 77,Sweden
2.IRCCS Istituto Clinico Humanitas,Rozzano, Milan,20089,Italy
3.Center for Research in Molecular Medicine & Chronic Diseases,Universidade de Santiago de Compostela,Santiago de Compostela,15782,Spain
4.Department of Biology and Biological Engineering,Chalmers University of Technology,Göteborg,412 96,Sweden
5.Max Delbrück Center for Molecular Medicine,Berlin,10115,Germany
6.Department of Biology,Southern University of Science and Technology,Shenzhen,518055,China
7.Department of Computer Science and Mathematics,Freie Universität Berlin,Berlin,14195,Germany
8.Statistics and Toxicology Section,Institute of Occupational Medicine,Edinburgh,EH14 4AP,United Kingdom

Keshavan，Sandeep,Andón，Fernando Torres,Gallud，Audrey,等. Profiling of sub‐lethal in vitro effects of multi‐walled carbon nanotubes reveals changes in chemokines and chemokine receptors[J]. Nanomaterials,2021,11(4).

Keshavan，Sandeep.,Andón，Fernando Torres.,Gallud，Audrey.,Chen，Wei.,Reinert，Knut.,...&Fadeel，Bengt.(2021).Profiling of sub‐lethal in vitro effects of multi‐walled carbon nanotubes reveals changes in chemokines and chemokine receptors.Nanomaterials,11(4).

Keshavan，Sandeep,et al."Profiling of sub‐lethal in vitro effects of multi‐walled carbon nanotubes reveals changes in chemokines and chemokine receptors".Nanomaterials 11.4(2021).