Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling

Ma，Zhiming1; Liu，Xiaolin1; Nath，Sangeeta2,6; Sun，He1; Tran，Tuan Minh1; Yang，Liang1,4,5; Mayor，Satyajit2,3; Miao，Yansong1

2021-03-30

10.1016/j.celrep.2021.108884

Cell Reports   影响因子和分区

2211-1247

Plants respond to bacterial infection acutely with actin remodeling during plant immune responses. The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic. Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv. campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI). Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale. By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling. Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.

actin nucleation actin remodeling diffusible signal factor formin nanoclustering PAMP PTI quorum sensing

SCI

英语

其他

WOS:000636315200002

2-s2.0-85103419548

Scopus

1.School of Biological Sciences,Nanyang Technological University,Singapore,637551,Singapore
2.Institute for Stem Cell Biology and Regenerative Medicine,Bangalore,Bellary Road,560065,India
3.National Centre for Biological Sciences,Tata Institute for Fundamental Research,Bangalore,Bellary Road,560065,India
4.Singapore Centre for Environmental Life Sciences Engineering,Nanyang Technological University,Singapore,637551,Singapore
5.School of Medicine,Southern University of Science and Technology,1088 Xueyuan Blvd.,Shenzhen,Nanshan District,518055,China
6.Manipal Institute of Regenerative Medicine,Manipal Academy of Higher Education,Bangalore,560065,India

Ma，Zhiming,Liu，Xiaolin,Nath，Sangeeta,et al. Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling[J]. Cell Reports,2021,34(13).

Ma，Zhiming.,Liu，Xiaolin.,Nath，Sangeeta.,Sun，He.,Tran，Tuan Minh.,...&Miao，Yansong.(2021).Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.Cell Reports,34(13).

Ma，Zhiming,et al."Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling".Cell Reports 34.13(2021).